Synthesis and Enzymatic Characterization of Sugar-Modified Nucleoside Triphosphate Analogs
Abstract
Chemical modification of nucleic acids can be achieved by the enzymatic polymerization of modified nucleoside triphosphates (dN*TPs). This approach obviates some of the requirements and drawbacks imposed by the more traditional solid-phase synthesis of oligonucleotides. Here, we describe the protocol that is necessary to synthesize dN*TPs and evaluate their substrate acceptance by polymerases for their subsequent use in various applications including selection experiments to identify aptamers. The protocol is exemplified for a sugar-constrained nucleoside analog, 7′,5′-bc-TTP.
Origin : Files produced by the author(s)
Licence : CC BY NC - Attribution - NonCommercial
Licence : CC BY NC - Attribution - NonCommercial