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Book Sections Year : 2019

Synthesis and Enzymatic Characterization of Sugar-Modified Nucleoside Triphosphate Analogs

Abstract

Chemical modification of nucleic acids can be achieved by the enzymatic polymerization of modified nucleoside triphosphates (dN*TPs). This approach obviates some of the requirements and drawbacks imposed by the more traditional solid-phase synthesis of oligonucleotides. Here, we describe the protocol that is necessary to synthesize dN*TPs and evaluate their substrate acceptance by polymerases for their subsequent use in various applications including selection experiments to identify aptamers. The protocol is exemplified for a sugar-constrained nucleoside analog, 7′,5′-bc-TTP.
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pasteur-03930944 , version 1 (09-01-2023)

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Attribution - NonCommercial

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Stella Diafa, Damien Evéquoz, Christian Leumann, Marcel Hollenstein. Synthesis and Enzymatic Characterization of Sugar-Modified Nucleoside Triphosphate Analogs. Non-Natural Nucleic Acids, 1973, Humana, pp.1-13, 2019, Methods in Molecular Biology, ⟨10.1007/978-1-4939-9216-4_1⟩. ⟨pasteur-03930944⟩
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