%0 Book Section %T Synthesis and Enzymatic Characterization of Sugar-Modified Nucleoside Triphosphate Analogs %+ University of Bern %+ Chimie bioorganique des acides nucléiques - Bioorganic chemistry of nucleic acids %A Diafa, Stella %A Evéquoz, Damien %A Leumann, Christian %A Hollenstein, Marcel %Z This work was supported by Institut Pasteur start-up funds to MH and the Swiss National Science Foundation (grant n°: 200020-146646). %B Non-Natural Nucleic Acids %I Humana %C New York, NY %S Methods in Molecular Biology %V 1973 %P 1-13 %8 2019-04-24 %D 2019 %R 10.1007/978-1-4939-9216-4_1 %M 31016692 %K Nucleoside triphosphate %K Primer extension reactions %K DNA polymerases %K XNA %K Solid-phase synthesis %K Bicyclo-DNA %Z Chemical Sciences %Z Chemical Sciences/Organic chemistryBook sections %X Chemical modification of nucleic acids can be achieved by the enzymatic polymerization of modified nucleoside triphosphates (dN*TPs). This approach obviates some of the requirements and drawbacks imposed by the more traditional solid-phase synthesis of oligonucleotides. Here, we describe the protocol that is necessary to synthesize dN*TPs and evaluate their substrate acceptance by polymerases for their subsequent use in various applications including selection experiments to identify aptamers. The protocol is exemplified for a sugar-constrained nucleoside analog, 7′,5′-bc-TTP. %G English %2 https://pasteur.hal.science/pasteur-03930944/document %2 https://pasteur.hal.science/pasteur-03930944/file/Book%20chapter.pdf %L pasteur-03930944 %U https://pasteur.hal.science/pasteur-03930944 %~ PASTEUR %~ CNRS