Cellular and molecular profiling of T-cell subsets at the onset of human acute GVHD - Institut Pasteur Access content directly
Journal Articles Blood Advances Year : 2020

Cellular and molecular profiling of T-cell subsets at the onset of human acute GVHD

Abstract

The cellular and molecular processes involved in acute graft-versus-host disease (aGVHD) development early after allogeneic hematopoietic cell transplantation (HCT) in humans remain largely unknown. We have performed multiparameter immunophenotyping and molecular profiling of CD4+ and CD8+ T cells in 2 independent cohorts of patients undergoing HCT, as well as in their HLA-identical sibling donors. Cellular profiling using spectral flow cytometry showed an incomplete reconstitution of the T-cell compartment in recipients without aGVHD early after transplantation, as well as a shift toward an effector memory phenotype, paralleled by depletion of the naive T-cell pool. Molecular profiling of T-cell populations in donors vs recipients without aGVHD revealed increased pathway activity of >40 gene modules in recipients. These pathways were associated in particular with T-cell activation, adhesion, migration, and effector functions. Cellular profiles from recipients developing aGVHD displayed an enrichment of cells with a T memory stem cell-like phenotype compared with recipients without aGVHD. Comparison of gene profiles from these recipients revealed that transforming growth factor-β (TGF-β) signaling was most significantly downregulated, whereas the pathway activity of NF-κB-associated transcription factors and signaling pathways were increased, at aGVHD onset. This study suggests that the integration of cellular and molecular profiles provides new insights into the development of aGVHD in humans.
Fichier principal
Vignette du fichier
advancesadv2019001032.pdf (1.95 Mo) Télécharger le fichier
Origin : Publisher files allowed on an open archive
Loading...

Dates and versions

pasteur-02918738 , version 1 (21-08-2020)

Identifiers

Cite

Eleonora Latis, David Michonneau, Claire Leloup, Hugo Varet, Régis Peffault de Latour, et al.. Cellular and molecular profiling of T-cell subsets at the onset of human acute GVHD. Blood Advances, 2020, 4 (16), pp.3927-3942. ⟨10.1182/bloodadvances.2019001032⟩. ⟨pasteur-02918738⟩
35 View
138 Download

Altmetric

Share

Gmail Facebook X LinkedIn More