Stability, affinity, and chromatic variants of the glutamate sensor iGluSnFR
Jonathan Marvin
(1)
,
Benjamin Scholl
(2)
,
Daniel Wilson
(2)
,
Kaspar Podgorski
(1)
,
Abbas Kazemipour
(1)
,
Johannes Alexander Müller
(3)
,
Susanne Schoch
(3)
,
Francisco José Urra Quiroz
(4)
,
Nelson Rebola
(4)
,
Huan Bao
(5)
,
Justin Little
(1)
,
Ariana Tkachuk
(1)
,
Edward Cai
(1)
,
Adam Hantman
(1)
,
Samuel S.-H. Wang
(6)
,
Victor Depiero
(7)
,
Bart Borghuis
(7)
,
Edwin Chapman
(5)
,
Dirk Dietrich
(3)
,
David Digregorio
(4)
,
David Fitzpatrick
(2)
,
Loren Looger
(1)
1
HHMI -
Howard Hughes Medical Institute
2 MPFI - Max Planck Florida Institute for Neuroscience
3 Rheinische Friedrich-Wilhelms-Universität Bonn
4 Dynamique des synapses et des circuits neuronaux - Synapse and circuit dynamics
5 University of Wisconsin-Madison
6 Princeton University
7 University of Louisville
2 MPFI - Max Planck Florida Institute for Neuroscience
3 Rheinische Friedrich-Wilhelms-Universität Bonn
4 Dynamique des synapses et des circuits neuronaux - Synapse and circuit dynamics
5 University of Wisconsin-Madison
6 Princeton University
7 University of Louisville
Jonathan Marvin
- Function : Author
- PersonId : 1252869
- ORCID : 0000-0003-2294-4515
Johannes Alexander Müller
- Function : Author
- PersonId : 1252870
- ORCID : 0000-0002-5578-6405
Bart Borghuis
- Function : Author
- PersonId : 1252871
- ORCID : 0000-0001-9867-4940
Loren Looger
Connectez-vous pour contacter l'auteur
- Function : Correspondent author
- PersonId : 1252922
Connectez-vous pour contacter l'auteur
Abstract
Single-wavelength fluorescent reporters allow visualization of specific neurotransmitters with high spatial and temporal resolution. We report variants of intensity-based glutamate-sensing fluorescent reporter (iGluSnFR) that are functionally brighter; detect submicromolar to millimolar amounts of glutamate; and have blue, cyan, green, or yellow emission profiles. These variants could be imaged in vivo in cases where original iGluSnFR was too dim, resolved glutamate transients in dendritic spines and axonal boutons, and allowed imaging at kilohertz rates.