%0 Journal Article %T Stability, affinity, and chromatic variants of the glutamate sensor iGluSnFR %+ Howard Hughes Medical Institute (HHMI) %+ Max Planck Florida Institute for Neuroscience (MPFI) %+ Rheinische Friedrich-Wilhelms-Universität Bonn %+ Dynamique des synapses et des circuits neuronaux - Synapse and circuit dynamics %+ University of Wisconsin-Madison %+ Princeton University %+ University of Louisville %A Marvin, Jonathan %A Scholl, Benjamin %A Wilson, Daniel %A Podgorski, Kaspar %A Kazemipour, Abbas %A Müller, Johannes Alexander %A Schoch, Susanne %A Quiroz, Francisco José Urra %A Rebola, Nelson %A Bao, Huan %A Little, Justin %A Tkachuk, Ariana %A Cai, Edward %A Hantman, Adam %A Wang, Samuel S.-H. %A Depiero, Victor %A Borghuis, Bart %A Chapman, Edwin %A Dietrich, Dirk %A Digregorio, David %A Fitzpatrick, David %A Looger, Loren %< avec comité de lecture %@ 1548-7091 %J Nature Methods %I Nature Publishing Group %V 15 %N 11 %P 936-939 %8 2018-11 %D 2018 %R 10.1038/s41592-018-0171-3 %M 30377363 %Z Life Sciences [q-bio]Journal articles %X Single-wavelength fluorescent reporters allow visualization of specific neurotransmitters with high spatial and temporal resolution. We report variants of intensity-based glutamate-sensing fluorescent reporter (iGluSnFR) that are functionally brighter; detect submicromolar to millimolar amounts of glutamate; and have blue, cyan, green, or yellow emission profiles. These variants could be imaged in vivo in cases where original iGluSnFR was too dim, resolved glutamate transients in dendritic spines and axonal boutons, and allowed imaging at kilohertz rates. %G English %L pasteur-04092458 %U https://pasteur.hal.science/pasteur-04092458 %~ PASTEUR %~ CNRS %~ SYN-CIR-DY %~ PASTEUR_UMR3571