Role of Klebsiella pneumoniae Type VI secretion system (T6SS) in long-term gastrointestinal colonization - Institut Pasteur Access content directly
Journal Articles Scientific Reports Year : 2022

Role of Klebsiella pneumoniae Type VI secretion system (T6SS) in long-term gastrointestinal colonization

Abstract

Abstract Type VI secretion systems (T6SS), recently described in hypervirulent K. pneumoniae (hvKp) strains , are involved in bacterial warfare but their role in classical clinical strains (cKp) has been little investigated . In silico analysis indicated the presence of T6SS clusters (from zero to four), irrespective of the strains origin or virulence, with a high prevalence in the K. pneumoniae species (98%). In the strain CH1157, two T6SS-apparented pathogenicity islands were detected, T6SS-1 and -2, harboring a phospholipase-encoding gene ( tle1 ) and a potential new effector-encoding gene named tke (Type VI Klebsiella effector). Tle1 expression in Escherichia coli periplasm affected cell membrane permeability. T6SS-1 isogenic mutants colonized the highest gastrointestinal tract of mice less efficiently than their parental strain, at long term. Comparative analysis of faecal 16S sequences indicated that T6SS-1 impaired the microbiota richness and its resilience capacity. Oscillospiraceae family members could be specific competitors for the long-term gut establishment of K. pneumoniae .
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pasteur-03809977 , version 1 (11-10-2022)

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Thomas Merciecca, Stéphanie Bornes, Laurence Nakusi, Sébastien Theil, Olaya Rendueles, et al.. Role of Klebsiella pneumoniae Type VI secretion system (T6SS) in long-term gastrointestinal colonization. Scientific Reports, 2022, 12 (1), pp.16968. ⟨10.1038/s41598-022-21396-w⟩. ⟨pasteur-03809977⟩
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