Large-scale Exploration of Growth Inhibition Caused by Overexpression of Genomic Fragments in Saccharomyces cerevisiae - Institut Pasteur Access content directly
Journal Articles Genome Biology Year : 2004

Large-scale Exploration of Growth Inhibition Caused by Overexpression of Genomic Fragments in Saccharomyces cerevisiae

Cécile Fairhead
  • Function : Author
  • PersonId : 910569
Emmanuelle Fabre
Gilles Fischer
Romain Koszul
Ingrid Lafontaine
Guy-Franck Richard

Abstract

We have screened the genome of Saccharomyces cerevisiae for fragments that confer a growth-retardation phenotype when overexpressed in a multicopy plasmid with a tetracycline-regulatable (Tet-off) promoter. We selected 714 such fragments with a mean size of 700 base-pairs out of around 84,000 clones tested. These include 493 in-frame open reading frame fragments corresponding to 454 distinct genes (of which 91 are of unknown function), and 162 out-of-frame, antisense and intergenic genomic fragments, representing the largest collection of toxic inserts published so far in yeast.
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pasteur-02868108 , version 1 (15-06-2020)

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Jeanne Boyer, Gwenaël Badis, Cécile Fairhead, Emmanuel Talla, Florence Hantraye, et al.. Large-scale Exploration of Growth Inhibition Caused by Overexpression of Genomic Fragments in Saccharomyces cerevisiae. Genome Biology, 2004, 5 (9), pp.R72. ⟨10.1186/gb-2004-5-9-r72⟩. ⟨pasteur-02868108⟩
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