%0 Journal Article %T Large-scale Exploration of Growth Inhibition Caused by Overexpression of Genomic Fragments in Saccharomyces cerevisiae %+ Génétique Moléculaire des Levures %+ Génétique des Interactions Macromoléculaires %+ Laboratoire de chimie bactérienne (LCB) %+ CHU Saint-Antoine [AP-HP] %+ Génétique et Biochimie du Développement %+ Génétique Moléculaire des Levures %A Boyer, Jeanne %A Badis, Gwenaël %A Fairhead, Cécile %A Talla, Emmanuel %A Hantraye, Florence %A Fabre, Emmanuelle %A Fischer, Gilles %A Hennequin, Christophe %A Koszul, Romain %A Lafontaine, Ingrid %A Ozier-Kalogeropoulos, Odile %A Ricchetti, Miria %A Richard, Guy-Franck %A Thierry, Agnès %A Dujon, Bernard %Z This work was supported in part by the EUROFAN2 project (Bio4-CT97-2294) from the European Commission (DGXII). E.T. was supported by the European contract CYGD (QLRI-CT 1999-01333), R.K. is a recipient of a CNRS-BDI fellowship. B.D. is a member of the Institut Universitaire de France. %< avec comité de lecture %@ 1465-6906 %J Genome Biology %I BioMed Central %V 5 %N 9 %P R72 %8 2004-08-31 %D 2004 %R 10.1186/gb-2004-5-9-r72 %M 15345056 %Z Life Sciences [q-bio]Journal articles %X We have screened the genome of Saccharomyces cerevisiae for fragments that confer a growth-retardation phenotype when overexpressed in a multicopy plasmid with a tetracycline-regulatable (Tet-off) promoter. We selected 714 such fragments with a mean size of 700 base-pairs out of around 84,000 clones tested. These include 493 in-frame open reading frame fragments corresponding to 454 distinct genes (of which 91 are of unknown function), and 162 out-of-frame, antisense and intergenic genomic fragments, representing the largest collection of toxic inserts published so far in yeast. %G English %2 https://pasteur.hal.science/pasteur-02868108/document %2 https://pasteur.hal.science/pasteur-02868108/file/document.pdf %L pasteur-02868108 %U https://pasteur.hal.science/pasteur-02868108 %~ PASTEUR %~ UPMC %~ CNRS %~ UNIV-AMU %~ APHP %~ SORBONNE-UNIVERSITE %~ SU-INF-2018 %~ SU-MEDECINE %~ SU-MED %~ SU-TI %~ ALLIANCE-SU %~ UMR3525