Intron retention-dependent gene regulation in Cryptococcus neoformans - Institut Pasteur Access content directly
Journal Articles Scientific Reports Year : 2016

Intron retention-dependent gene regulation in Cryptococcus neoformans

Damien Paulet
  • Function : Author
  • PersonId : 902433
Jean-Yves Coppée

Abstract

The biological impact of alternative splicing is poorly understood in fungi, although recent studies have shown that these microorganisms are usually intron-rich. In this study, we re-annotated the genome of C. neoformans var. neoformans using RNA-Seq data. Comparison with C. neoformans var. grubii revealed that more than 99% of ORF-introns are in the same exact position in the two varieties whereas UTR-introns are much less evolutionary conserved. We also confirmed that alternative splicing is very common in C. neoformans, affecting nearly all expressed genes. We also observed specific regulation of alternative splicing by environmental cues in this yeast. However, alternative splicing does not appear to be an efficient method to diversify the C. neoformans proteome. Instead, our data suggest the existence of an intron retention-dependent mechanism of gene expression regulation that is not dependent on NMD. This regulatory process represents an additional layer of gene expression regulation in fungi and provides a mechanism to tune gene expression levels in response to any environmental modification.
Fichier principal
Vignette du fichier
Intron retention-dependent gene regulation in Cryptococcus neoformans.pdf (4.11 Mo) Télécharger le fichier
Origin Publication funded by an institution
Loading...

Dates and versions

pasteur-02861773 , version 1 (09-06-2020)

Licence

Identifiers

Cite

Sara Gonzalez-Hilarion, Damien Paulet, Kyung-Tae Lee, Chung-Chau Hon, Pierre Lechat, et al.. Intron retention-dependent gene regulation in Cryptococcus neoformans. Scientific Reports, 2016, 6 (1), pp.32252. ⟨10.1038/srep32252⟩. ⟨pasteur-02861773⟩
25 View
64 Download

Altmetric

Share

Gmail Mastodon Facebook X LinkedIn More