Essential dynamic interdependence of FtsZ and SepF for Z-ring and septum formation in Corynebacterium glutamicum - Institut Pasteur Access content directly
Journal Articles Nature Communications Year : 2020

Essential dynamic interdependence of FtsZ and SepF for Z-ring and septum formation in Corynebacterium glutamicum

Abstract

The mechanisms of Z-ring assembly and regulation in bacteria are poorly understood, particularly in non-model organisms. Actinobacteria, a large bacterial phylum that includes the pathogen Mycobacterium tuberculosis, lack the canonical FtsZ-membrane anchors and Z-ring regulators described for E. coli. Here we investigate the physiological function of Cor-ynebacterium glutamicum SepF, the only cell division-associated protein from Actinobacteria known to interact with the conserved C-terminal tail of FtsZ. We show an essential interdependence of FtsZ and SepF for formation of a functional Z-ring in C. glutamicum. The crystal structure of the SepF-FtsZ complex reveals a hydrophobic FtsZ-binding pocket, which defines the SepF homodimer as the functional unit, and suggests a reversible oligomerization interface. FtsZ filaments and lipid membranes have opposing effects on SepF polymerization, indicating that SepF has multiple roles at the cell division site, involving FtsZ bundling, Z-ring tethering and membrane reshaping activities that are needed for proper Z-ring assembly and function.
Fichier principal
Vignette du fichier
s41467-020-15490-8.pdf (3.75 Mo) Télécharger le fichier
Origin Publication funded by an institution
Loading...

Dates and versions

pasteur-02559164 , version 1 (30-04-2020)

Licence

Identifiers

Cite

Adrià Sogues, Mariano Martinez, Quentin Gaday, Mathilde Ben Assaya, Martín Graña, et al.. Essential dynamic interdependence of FtsZ and SepF for Z-ring and septum formation in Corynebacterium glutamicum. Nature Communications, 2020, 11 (1), pp.1641. ⟨10.1038/s41467-020-15490-8⟩. ⟨pasteur-02559164⟩
185 View
121 Download

Altmetric

Share

Gmail Mastodon Facebook X LinkedIn More