A New High-Throughput Tool to Screen Mosquito-Borne Viruses in Zika Virus Endemic/Epidemic Areas - Institut Pasteur Access content directly
Journal Articles Viruses Year : 2019

A New High-Throughput Tool to Screen Mosquito-Borne Viruses in Zika Virus Endemic/Epidemic Areas

Anubis Vega-Rúa
Sandrine Chantilly
  • Function : Author
  • PersonId : 1056583
Johana Restrepo
  • Function : Author
  • PersonId : 1056584
Isabelle Dusfour
Mawlouth Diallo
  • Function : Author
  • PersonId : 1056586
Alioune Gaye
  • Function : Author
  • PersonId : 1056587
Sébastien Boyer
Veasna Duong
Géraldine Piorkowski
  • Function : Author
  • PersonId : 1056588
Xavier de Lamballerie
  • Function : Author
  • PersonId : 1056589

Abstract

Mosquitoes are vectors of arboviruses affecting animal and human health. Arboviruses circulate primarily within an enzootic cycle and recurrent spillovers contribute to the emergence of human-adapted viruses able to initiate an urban cycle involving anthropophilic mosquitoes. The increasing volume of travel and trade offers multiple opportunities for arbovirus introduction in new regions. This scenario has been exemplified recently with the Zika pandemic. To incriminate a mosquito as vector of a pathogen, several criteria are required such as the detection of natural infections in mosquitoes. In this study, we used a high-throughput chip based on the BioMark™ Dynamic arrays system capable of detecting 64 arboviruses in a single experiment. A total of 17,958 mosquitoes collected in Zika-endemic/epidemic countries (Brazil, French Guiana, Guadeloupe, Suriname, Senegal, and Cambodia) were analyzed. Here we show that this new tool can detect endemic and epidemic viruses in different mosquito species in an epidemic context. Thus, this fast and low-cost method can be suggested as a novel epidemiological surveillance tool to identify circulating arboviruses.
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pasteur-02325649 , version 1 (22-10-2019)

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Sara Moutailler, Léna Yousfi, Laurence Mousson, Elodie Devillers, Marie Vazeille, et al.. A New High-Throughput Tool to Screen Mosquito-Borne Viruses in Zika Virus Endemic/Epidemic Areas. Viruses, 2019, 11 (10), pp.904. ⟨10.3390/v11100904⟩. ⟨pasteur-02325649⟩
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