β-lactam antibiotics promote bacterial mutagenesis via an RpoS-mediated reduction in replication fidelity - Institut Pasteur Access content directly
Journal Articles Nature Communications Year : 2013

β-lactam antibiotics promote bacterial mutagenesis via an RpoS-mediated reduction in replication fidelity

Abstract

Regardless of their targets and modes of action, subinhibitory concentrations of antibiotics can have an impact on cell physiology and trigger a large variety of cellular responses in different bacterial species. Subinhibitory concentrations of β-lactam antibiotics cause reactive oxygen species production and induce PolIV-dependent mutagenesis in Escherichia coli. Here we show that subinhibitory concentrations of β-lactam antibiotics induce the RpoS regulon. RpoS regulon induction is required for PolIV-dependent mutagenesis because it diminishes the control of DNA-replication fidelity by depleting MutS in E. coli, Vibrio cholerae and Pseudomonas aeruginosa. We also show that in E. coli, the reduction in mismatch-repair activity is mediated by SdsR, the RpoS-controlled small RNA. In summary, we show that mutagenesis induced by subinhibitory concentrations of antibiotics is a genetically controlled process. Because this mutagenesis can generate mutations conferring antibiotic resistance, it should be taken into consideration for the development of more efficient antimicrobial therapeutic strategies.
Fichier principal
Vignette du fichier
Nat Commun_2607.pdf (675.76 Ko) Télécharger le fichier
Origin : Publication funded by an institution
Loading...

Dates and versions

pasteur-01691736 , version 1 (24-01-2018)

Licence

Attribution - NonCommercial - ShareAlike

Identifiers

Cite

A. Gutierrez, L. Laureti, S. Crussard, H. Abida, A. Rodríguez-Rojas, et al.. β-lactam antibiotics promote bacterial mutagenesis via an RpoS-mediated reduction in replication fidelity. Nature Communications, 2013, 4, pp.1610. ⟨10.1038/ncomms2607⟩. ⟨pasteur-01691736⟩
364 View
240 Download

Altmetric

Share

Gmail Facebook X LinkedIn More