Molecular identification of Mucorales in human tissues: contribution of PCR electrospray-ionization mass spectrometry - Institut Pasteur Access content directly
Journal Articles Clinical Microbiology and Infection Year : 2015

Molecular identification of Mucorales in human tissues: contribution of PCR electrospray-ionization mass spectrometry

B. Denis
  • Function : Author
French Mycosis Study Group
  • Function : Author

Abstract

Molecular methods are crucial for mucormycosis diagnosis because cultures are frequently negative, even if microscopy suggests the presence of hyphae in tissues. We assessed PCR/electrospray-ionization mass spectrometry (PCR/ESI-MS) for Mucorales identification in 19 unfixed tissue samples from 13 patients with proven or probable mucormycosis and compared the results with culture, quantitative real-time PCR, 16S-23S rRNA gene internal transcribed spacer region (ITS PCR) and 18S PCR sequencing. Concordance with culture identification to both genus and species levels was higher for PCR/ESI-MS than for the other techniques. Thus, PCR/ESI-MS is suitable for Mucorales identification, within 6 hours, for tissue samples for which microscopy results suggest the presence of hyphae.

Dates and versions

pasteur-01405221 , version 1 (29-11-2016)

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Alexandre Alanio, D. Garcia-Hermoso, S. Mercier-Delarue, F. Lanternier, M. Gits-Muselli, et al.. Molecular identification of Mucorales in human tissues: contribution of PCR electrospray-ionization mass spectrometry. Clinical Microbiology and Infection, 2015, 21 (6), pp.594.e1--5. ⟨10.1016/j.cmi.2015.01.017⟩. ⟨pasteur-01405221⟩
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