Molecular identification of Mucorales in human tissues: contribution of PCR electrospray-ionization mass spectrometry
Résumé
Molecular methods are crucial for mucormycosis diagnosis because cultures are frequently negative, even if microscopy suggests the presence of hyphae in tissues. We assessed PCR/electrospray-ionization mass spectrometry (PCR/ESI-MS) for Mucorales identification in 19 unfixed tissue samples from 13 patients with proven or probable mucormycosis and compared the results with culture, quantitative real-time PCR, 16S-23S rRNA gene internal transcribed spacer region (ITS PCR) and 18S PCR sequencing. Concordance with culture identification to both genus and species levels was higher for PCR/ESI-MS than for the other techniques. Thus, PCR/ESI-MS is suitable for Mucorales identification, within 6 hours, for tissue samples for which microscopy results suggest the presence of hyphae.
Mots clés
Culture
DNA
Fungal
electrospray-ionization mass spectrometry
Humans
Microbiological Techniques
Mucorales
Mucorales identification
Mucormycosis
Pathology
Molecular
PCR
Polymerase Chain Reaction
Prospective Studies
Real-Time Polymerase Chain Reaction
Sequence Analysis
species level
Spectrometry
Mass
Electrospray Ionization
Time Factors