Protocol for live imaging of bacteria-cell interactions in genetically modified mouse small intestinal organoids
Résumé
Here, we present a protocol for microinjection of bacteria into mouse small intestinal organoids that recapitulates the natural route of infection of intestinal epithelial cells from the intestinal lumen. We describe steps for visualizing bacteria-cell interactions by live imaging of infected organoids using light sheet microscopy. We then detail procedures for generating doxycycline-inducible expression of mutant proteins in organoids to study essential gene functions. The different techniques described in this protocol can be used independently as required.
For complete details on the use and execution of this protocol, please refer to Kim et al. (2021)
Domaines
Sciences du Vivant [q-bio]
Fichier principal
Revised MS_101123.pdf (838.86 Ko)
Télécharger le fichier
Figures_1. Preparation of the organoids for microinjection.jpg (1.18 Mo)
Télécharger le fichier
Figures_2. Image of the microinjection system.jpg (1.06 Mo)
Télécharger le fichier
Figures_3. Microinjection of the organoids.jpg (997.21 Ko)
Télécharger le fichier
Figures_4. Transduction of the organoids.jpg (328.7 Ko)
Télécharger le fichier
STAR_GA.jpg (138.04 Ko)
Télécharger le fichier
| Origine | Fichiers produits par l'(les) auteur(s) |
|---|---|
| Licence |
| Format | Figure, Image |
|---|---|
| Licence |
| Format | Figure, Image |
|---|---|
| Licence |
| Format | Figure, Image |
|---|---|
| Licence |
| Format | Figure, Image |
|---|---|
| Licence |
| Format | Figure, Image |
|---|---|
| Licence |