Protocol for separation of fungal extracellular vesicles using ultracentrifugation from solid medium cultures - Institut Pasteur Accéder directement au contenu
Article Dans Une Revue STAR Protocols Année : 2024

Protocol for separation of fungal extracellular vesicles using ultracentrifugation from solid medium cultures

Résumé

Extracellular vesicles (EVs) have been identified in diverse fungi, including human pathogens. In this protocol, we present two techniques for isolating and analyzing fungal EVs. The first is for high-throughput screening, and the second is for yielding concentrated samples suitable for centrifugation-based density gradients. We describe steps for analytical assays such as nano-flow cytometry and nanoparticle tracking analysis to measure EV dimensions and concentration. EV suspensions can serve diverse assays, including electron microscopy, compositional determination, and cell-to-cell communication assays.

Domaines

Mycologie
Fichier principal
Vignette du fichier
main.pdf (5.47 Mo) Télécharger le fichier
Origine Publication financée par une institution
Licence

Dates et versions

pasteur-04602213 , version 1 (05-06-2024)

Licence

Identifiants

Citer

Alicia C Piffer, Flavia C.G. Reis, Frédérique Moyrand, Bruna Montalvão, Marcio L Rodrigues, et al.. Protocol for separation of fungal extracellular vesicles using ultracentrifugation from solid medium cultures. STAR Protocols, 2024, 5 (2), pp.103069. ⟨10.1016/j.xpro.2024.103069⟩. ⟨pasteur-04602213⟩

Collections

PASTEUR RIIP ANR
15 Consultations
11 Téléchargements

Altmetric

Partager

Gmail Mastodon Facebook X LinkedIn More