Interplay of intracellular and trans‐cellular DNA methylation in natural archaeal consortia - Institut Pasteur Accéder directement au contenu
Article Dans Une Revue Environmental Microbiology Reports Année : 2024

Interplay of intracellular and trans‐cellular DNA methylation in natural archaeal consortia

Résumé

DNA methylation serves a variety of functions across all life domains. In this study, we investigated archaeal methylomics within a tripartite xylanolytic halophilic consortium. This consortium includes Haloferax lucertense SVX82, Halorhabdus sp. SVX81, and an ectosymbiotic Candidatus Nanohalococcus occultus SVXNc, a nano‐sized archaeon from the DPANN superphylum. We utilized PacBio SMRT and Illumina cDNA sequencing to analyse samples from consortia of different compositions for methylomics and transcriptomics. Endogenous c TAG methylation, typical of Haloferax , was accompanied in this strain by methylation at four other motifs, including GDG c HC methylation, which is specific to the ectosymbiont. Our analysis of the distribution of methylated and unmethylated motifs suggests that autochthonous cTAG methylation may influence gene regulation. The frequency of GRAGA a G methylation increased in highly expressed genes, while C c TTG and GTCG a GG methylation could be linked to restriction‐modification (RM) activity. Generally, the RM activity might have been reduced during the evolution of this archaeon to balance the protection of cells from intruders, the reduction of DNA damage due to self‐restriction in stressful environments, and the benefits of DNA exchange under extreme conditions. Our methylomics, transcriptomics and complementary electron cryotomography (cryo‐ET) data suggest that the nanohaloarchaeon exports its methyltransferase to methylate the Haloferax genome, unveiling a new aspect of the interaction between the symbiont and its host.
Fichier principal
Vignette du fichier
Reva2024EnvironMicrobiolRep.pdf (16.3 Mo) Télécharger le fichier
Origine : Publication financée par une institution
Licence : CC BY - Paternité

Dates et versions

pasteur-04539987 , version 1 (10-04-2024)

Licence

Paternité

Identifiants

Citer

Oleg N Reva, Violetta La Cono, Francesca Crisafi, Francesco Smedile, Manasi Mudaliyar, et al.. Interplay of intracellular and trans‐cellular DNA methylation in natural archaeal consortia. Environmental Microbiology Reports, 2024, 16 (2), pp.e13258. ⟨10.1111/1758-2229.13258⟩. ⟨pasteur-04539987⟩

Collections

PASTEUR CNRS ANR
13 Consultations
4 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More