Apical Invasion of Intestinal Epithelial Cells by Salmonella typhimurium Requires Villin to Remodel the Brush Border Actin Cytoskeleton - Institut Pasteur Accéder directement au contenu
Article Dans Une Revue Cell Host & Microbe Année : 2015

Apical Invasion of Intestinal Epithelial Cells by Salmonella typhimurium Requires Villin to Remodel the Brush Border Actin Cytoskeleton

Résumé

Salmonella invasion of intestinal epithelial cells requires extensive, though transient, actin modifications at the site of bacterial entry. The actin-modifying protein villin is present in the brush border where it participates in the constitution of microvilli and in epithelial restitution after damage through its actin-severing activity. We investigated a possible role for villin in Salmonella invasion. The absence of villin, which is normally located at the bacterial entry site, leads to a decrease in Salmonella invasion. Villin is necessary for early membrane-associated processes and for optimal ruffle assembly by balancing the steady-state level of actin. The severing activity of villin is important for Salmonella invasion in vivo. The bacterial phosphatase SptP tightly regulates villin phosphorylation, while the actin-binding effector SipA protects F-actin and counterbalances villin-severing activity. Thus, villin plays an important role in establishing the balance between actin polymerization and actin severing to facilitate the initial steps of Salmonella entry.
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Licence : CC BY NC ND - Paternité - Pas d'utilisation commerciale - Pas de modification

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pasteur-04379953 , version 1 (08-01-2024)

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Paternité - Pas d'utilisation commerciale - Pas de modification

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Nouara Lhocine, Ellen T. Arena, Perrine Bomme, Florent Ubelmann, Marie-Christine Prévost, et al.. Apical Invasion of Intestinal Epithelial Cells by Salmonella typhimurium Requires Villin to Remodel the Brush Border Actin Cytoskeleton. Cell Host & Microbe, 2015, 17 (2), pp.164-177. ⟨10.1016/j.chom.2014.12.003⟩. ⟨pasteur-04379953⟩
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