Exploring the Archaeal Virosphere by Metagenomics - Institut Pasteur Access content directly
Book Sections Year : 2024

Exploring the Archaeal Virosphere by Metagenomics


During the past decade, environmental research has demonstrated that archaea are abundant and widespread in nature, and play important ecological roles at a global scale. Currently, however, the majority of archaeal lineages cannot be cultivated under laboratory conditions and are known exclusively or nearly exclusively through metagenomics. A similar trend extends to the archaeal virosphere, where isolated representatives are available for a handful of model archaeal virus-host systems. Viral metagenomics provides an alternative way to circumvent the limitations of culture-based virus discovery and offers insight into the diversity, distribution and environmental impact of uncultured archaeal viruses. Presently, metagenomics approaches have been successfully applied to exploring the viromes associated with various lineages of extremophilic and mesophilic archaea, including Asgard archaea (Asgardarchaeota), ANME-1 archaea (Methanophagales), thaumarchaea (Nitrososphaeria), altiarchaea (Altiarchaeota) and marine group II archaea (Poseidoniales). Here, we provide an overview of methods widely used in archaeal virus metagenomics, covering metavirome preparation, genome annotation, phylogenetic and phylogenomic analyses, and archaeal host assignment. We hope that this summary will contribute to further exploration and characterization of the enigmatic archaeal virome lurking in diverse environments.
Embargoed file
Embargoed file
0 3 11
Year Month Jours
Avant la publication
Saturday, June 8, 2024
Embargoed file
Saturday, June 8, 2024
Please log in to request access to the document

Dates and versions

pasteur-04330156 , version 1 (07-12-2023)


Attribution - NonCommercial



Yifan Zhou, Yongjie Wang, David Prangishvili, Mart Krupovic. Exploring the Archaeal Virosphere by Metagenomics. Viral Metagenomics, 2732, Humana, pp.1-22, 2024, Methods in Molecular Biology, 978-1-0716-3514-8. ⟨10.1007/978-1-0716-3515-5_1⟩. ⟨pasteur-04330156⟩
117 View
4 Download



Gmail Facebook X LinkedIn More