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Pré-Publication, Document De Travail Année : 2023

The host RNA polymerase II C-terminal domain is the anchor for replication of the influenza virus genome

Résumé

Summary The current model is that the influenza virus polymerase (FluPol) binds either to host RNA polymerase II (RNAP II) or to the acidic nuclear phosphoprotein 32 (ANP32), which drives its conformation and activity towards transcription or replication of the viral genome, respectively. Here, we provide evidence that the FluPol-RNAP II binding interface has a so far overlooked function for replication of the viral genome. Using a combination of cell-based and in vitro approaches, we show that the RNAP II C-terminal-domain, jointly with ANP32, enhances FluPol replication activity and we propose a model in which the host RNAP II is the anchor for transcription and replication of the viral genome. Our data open new perspectives on the spatial coupling of viral transcription and replication and the coordinated balance between these two activities.
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Licence : CC BY NC ND - Paternité - Pas d'utilisation commerciale - Pas de modification

Dates et versions

pasteur-04269644 , version 1 (03-11-2023)

Licence

Paternité - Pas d'utilisation commerciale - Pas de modification

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Tim Krischuns, B Arragain, C Isel, Sylvain Paisant, M Budt, et al.. The host RNA polymerase II C-terminal domain is the anchor for replication of the influenza virus genome. 2023. ⟨pasteur-04269644⟩
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