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Article Dans Une Revue (Data Paper) International Journal of Systematic and Evolutionary Microbiology Année : 2022

Sutcliffiella rhizosphaerae sp. nov. isolated from roots

Résumé

An aerobic, Gram-staining-positive, endospore-forming bacterium, isolated from the rhizosphere of roots of maize ( Zea mays ), was taxonomically studied. On the basis of 16S rRNA gene sequence similarity comparisons, strain JJ-125 T clustered together with species of the genus Sutcliffiella and showed the highest similarities with Sutcliffiella zhanjiangensis (98.7 %). The 16S rRNA gene sequence similarities to the sequences of the type strains of other species of the genus Sutcliffiella were <98.4 %. The genome sequence of JJ-125 T was 4 516 360 bp long and had a DNA G+C content of 37.3 %. A DNA–DNA hybridization with the type strain of S. zhanjiangensis DSM 23010 T resulted in values of 42.3 and 43.9 % (reciprocal). The average nucleotide identity, average amino acid identity and digital DNA–DNA hybridization values between the JJ-125 T genome assembly and those of the other type strains of species of the genus Sutcliffiella were <75%, <80 % and <21 %, respectively. Chemotaxonomic features supported the grouping of the strain with the genus Sutcliffiella, e.g. the major fatty acids included iso-C 15 : 0, iso-C 17 : 1 ω10 c and iso-C 17 : 0 , the polar lipid profile contained the major components diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine, the only quinone was menaquinone MK-7 and the characteristic diamino acid was meso -diaminopimelic acid. Physiological and biochemical test results were also different from those of the most closely related species. As a consequence, JJ-125 T represents a novel species of the genus Sutcliffiella , for which we propose the name Sutcliffiella rhizosphaerae sp. nov., with JJ-125 T (= CIP 111883 T = LMG 32156 T = CCM 9046 T ) as the type strain.
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Dates et versions

pasteur-04109954 , version 1 (30-05-2023)

Identifiants

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Peter Kämpfer, Stefanie P Glaeser, André Lipski, John A Mcinroy, D. Clermont, et al.. Sutcliffiella rhizosphaerae sp. nov. isolated from roots. International Journal of Systematic and Evolutionary Microbiology, 2022, 72 (10), pp.005590. ⟨10.1099/ijsem.0.005590⟩. ⟨pasteur-04109954⟩
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