A reference map of the human binary protein interactome - Institut Pasteur Access content directly
Journal Articles Nature Year : 2020

A reference map of the human binary protein interactome

Katja Luck (1, 2) , Dae-Kyum Kim , Luke Lambourne , Kerstin Spirohn , Bridget Begg , Wenting Bian , Ruth Brignall , Tiziana Cafarelli , Francisco Campos-Laborie , Benoit Charloteaux , Dongsic Choi , Atina Coté , Meaghan Daley , Steven Deimling , Alice Desbuleux , Amélie Dricot , Marinella Gebbia , Madeleine Hardy , Nishka Kishore , Jennifer Knapp , István Kovács , Irma Lemmens , Miles Mee , Joseph Mellor , Carl Pollis , Carles Pons , Aaron Richardson , Sadie Schlabach , Bridget Teeking , Anupama Yadav , Mariana Babor , Dawit Balcha , Omer Basha , Christian Bowman-Colin , Suet-Feung Chin , Soon Gang Choi , Claudia Colabella , Georges Coppin , Cassandra D’amata , David de Ridder , Steffi de Rouck , Miquel Duran-Frigola , Hanane Ennajdaoui , Florian Goebels , Liana Goehring , Anjali Gopal , Ghazal Haddad , Elodie Hatchi , Mohamed Helmy , Yves Jacob (3) , Yoseph Kassa , Serena Landini , Roujia Li , Natascha van Lieshout , Andrew Macwilliams , Dylan Markey , Joseph Paulson , Sudharshan Rangarajan , John Rasla , Ashyad Rayhan , Thomas Rolland , Adriana San-Miguel , Yun Shen , Dayag Sheykhkarimli , Gloria Sheynkman , Eyal Simonovsky , Murat Taşan , Alexander Tejeda , Vincent Tropepe , Jean-Claude Twizere , Yang Wang , Robert Weatheritt , Jochen Weile , Yu Xia , Xinping Yang , Esti Yeger-Lotem , Quan Zhong , Patrick Aloy , Gary Bader , Javier de las Rivas , Suzanne Gaudet , Tong Hao , Janusz Rak , Jan Tavernier , David Hill (1, 2) , Marc Vidal (1, 2) , Frederick Roth (1, 4, 5, 6) , Michael Calderwood (1, 2)
Dae-Kyum Kim
  • Function : Author
Luke Lambourne
Kerstin Spirohn
Bridget Begg
  • Function : Author
Wenting Bian
  • Function : Author
Ruth Brignall
  • Function : Author
Tiziana Cafarelli
  • Function : Author
Francisco Campos-Laborie
  • Function : Author
Benoit Charloteaux
  • Function : Author
Dongsic Choi
  • Function : Author
Atina Coté
  • Function : Author
Meaghan Daley
  • Function : Author
Steven Deimling
  • Function : Author
Alice Desbuleux
  • Function : Author
Amélie Dricot
  • Function : Author
Marinella Gebbia
  • Function : Author
Madeleine Hardy
  • Function : Author
Nishka Kishore
  • Function : Author
Jennifer Knapp
  • Function : Author
István Kovács
  • Function : Author
Irma Lemmens
  • Function : Author
Miles Mee
  • Function : Author
Joseph Mellor
  • Function : Author
Carl Pollis
  • Function : Author
Carles Pons
  • Function : Author
Aaron Richardson
  • Function : Author
Sadie Schlabach
  • Function : Author
Bridget Teeking
  • Function : Author
Anupama Yadav
Mariana Babor
  • Function : Author
Dawit Balcha
  • Function : Author
Omer Basha
  • Function : Author
Christian Bowman-Colin
  • Function : Author
Suet-Feung Chin
Soon Gang Choi
Claudia Colabella
Georges Coppin
  • Function : Author
Cassandra D’amata
  • Function : Author
David de Ridder
  • Function : Author
Steffi de Rouck
  • Function : Author
Miquel Duran-Frigola
  • Function : Author
Hanane Ennajdaoui
  • Function : Author
Florian Goebels
  • Function : Author
Liana Goehring
  • Function : Author
Anjali Gopal
  • Function : Author
Ghazal Haddad
  • Function : Author
Elodie Hatchi
  • Function : Author
Mohamed Helmy
Yoseph Kassa
  • Function : Author
Serena Landini
  • Function : Author
Roujia Li
  • Function : Author
Natascha van Lieshout
  • Function : Author
Andrew Macwilliams
  • Function : Author
Dylan Markey
  • Function : Author
Joseph Paulson
Sudharshan Rangarajan
  • Function : Author
John Rasla
  • Function : Author
Ashyad Rayhan
  • Function : Author
Thomas Rolland
  • Function : Author
Adriana San-Miguel
  • Function : Author
Yun Shen
  • Function : Author
Dayag Sheykhkarimli
Gloria Sheynkman
  • Function : Author
Eyal Simonovsky
  • Function : Author
Murat Taşan
  • Function : Author
Alexander Tejeda
  • Function : Author
Vincent Tropepe
  • Function : Author
Jean-Claude Twizere
Yang Wang
  • Function : Author
Robert Weatheritt
  • Function : Author
Jochen Weile
  • Function : Author
Yu Xia
Xinping Yang
  • Function : Author
Esti Yeger-Lotem
Quan Zhong
Patrick Aloy
  • Function : Author
Gary Bader
Javier de las Rivas
Suzanne Gaudet
  • Function : Author
Tong Hao
Janusz Rak
Jan Tavernier
  • Function : Author

Abstract

Global insights into cellular organization and genome function require comprehensive understanding of the interactome networks that mediate genotype–phenotype relationships1,2. Here we present a human ‘all-by-all’ reference interactome map of human binary protein interactions, or ‘HuRI’. With approximately 53,000 protein–protein interactions, HuRI has approximately four times as many such interactions as there are high-quality curated interactions from small-scale studies. The integration of HuRI with genome3, transcriptome4 and proteome5 data enables cellular function to be studied within most physiological or pathological cellular contexts. We demonstrate the utility of HuRI in identifying the specific subcellular roles of protein–protein interactions. Inferred tissue-specific networks reveal general principles for the formation of cellular context-specific functions and elucidate potential molecular mechanisms that might underlie tissue-specific phenotypes of Mendelian diseases. HuRI is a systematic proteome-wide reference that links genomic variation to phenotypic outcomes.

Dates and versions

pasteur-04098559 , version 1 (16-05-2023)

Identifiers

Cite

Katja Luck, Dae-Kyum Kim, Luke Lambourne, Kerstin Spirohn, Bridget Begg, et al.. A reference map of the human binary protein interactome. Nature, 2020, 580 (7803), pp.402-408. ⟨10.1038/s41586-020-2188-x⟩. ⟨pasteur-04098559⟩
23 View
0 Download

Altmetric

Share

Gmail Facebook X LinkedIn More