Flowcytometric and ImageStream RNA-FISH Gene Expression, Quantification and Phenotypic Characterization of Blood Stages and Sporozoites From Human Malaria Species - Institut Pasteur Access content directly
Journal Articles Journal of Infectious Diseases Year : 2022

Flowcytometric and ImageStream RNA-FISH Gene Expression, Quantification and Phenotypic Characterization of Blood Stages and Sporozoites From Human Malaria Species

Abstract

We adapted the RNA FISH Stellaris method to specifically detect the expression of Plasmodium genes by flow cytometry and ImageStream (Flow-FISH). This new method accurately quantified the erythrocytic forms of (1) Plasmodium falciparum and Plasmodium vivax and (2) the sexual stages of P vivax from patient isolates. ImageStream analysis of liver stage sporozoites using a combination of surface circumsporozoite protein (CSP), deoxyribonucleic acid, and 18S RNA labeling proved that the new Flow-FISH is suitable for gene expression studies of transmission stages. This powerful multiparametric single-cell method offers a platform of choice for both applied and fundamental research on the biology of malaria parasites.
Fichier principal
Vignette du fichier
jiab431.pdf (3.58 Mo) Télécharger le fichier
Origin Publication funded by an institution

Dates and versions

pasteur-03727545 , version 1 (19-07-2022)

Licence

Identifiers

Cite

Camilla Luiza-Batista, Flore Nardella, Sabine Thiberge, Malika Serra-Hassoun, Marcelo U. Ferreira, et al.. Flowcytometric and ImageStream RNA-FISH Gene Expression, Quantification and Phenotypic Characterization of Blood Stages and Sporozoites From Human Malaria Species. Journal of Infectious Diseases, 2022, 225 (9), pp.1621-1625. ⟨10.1093/infdis/jiab431⟩. ⟨pasteur-03727545⟩
43 View
72 Download

Altmetric

Share

Gmail Mastodon Facebook X LinkedIn More