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Journal Articles Nucleic Acids Research Year : 1990

DNA recombination during PCR

Abstract

PCR co-amplification of two distinct HIV1 tat gene sequences lead to the formation of recombinant DNA molecules. The frequency of such recombinants, up to 5.4% of all amplified molecules, could be decreased 2.7 fold by a 6 fold increase in Taq DNA polymerase elongation time. Crossover sites mapped essentially to three discrete regions suggesting specific Taq DNA polymerase pause or termination sites. PCR mediated recombination may be a problem when studying heterogeneous genetic material such as RNA viruses, multigene families, or repetitive sequences. This phenomenon can be exploited to create chimeric molecules from related sequences.

Dates and versions

pasteur-03520139 , version 1 (10-01-2022)

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Andreas Meyerhans, Jean Pierre Vartanian, Simon Wain-Hobson. DNA recombination during PCR. Nucleic Acids Research, 1990, 18 (7), pp.1687-1691. ⟨10.1093/nar/18.7.1687⟩. ⟨pasteur-03520139⟩

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