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Journal Articles Bio-protocol Year : 2017

Measurement of Dipeptidylpeptidase Activity in vitro and in vivo

Abstract

Dipeptidylpeptidases (DPPs) are serine proteases, which cleave small proteins and peptides possessing a proline or an alanine in the second position of their N-terminus. Among the members of this family, dipeptidylpeptidase 4 (DPP4) is constitutively expressed in the extracellular space. DPP4 is found at the surface of many hematopoietic and non-hematopoietic cells and is also present in many biological fluids in a bioactive soluble form. DPP4 expression is modulated by inflammation, and measurements of its activity have been used as biomarker for disease. Here, we describe a method to evaluate the enzymatic activity of DPP4 in vitro and in vivo.

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Immunology
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pasteur-03331047 , version 1 (01-09-2021)

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Rosa Barreira da Silva, Molly Ingersoll, Matthew Albert. Measurement of Dipeptidylpeptidase Activity in vitro and in vivo. Bio-protocol , 2017, 7 (6), pp.e2184. ⟨10.21769/BioProtoc.2184⟩. ⟨pasteur-03331047⟩

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