Protein F. A novel F(ab)-binding factor, present in normal liver, and largely released in the digestive tract during hepatitis - Institut Pasteur Access content directly
Journal Articles Journal of Immunology Year : 1990

Protein F. A novel F(ab)-binding factor, present in normal liver, and largely released in the digestive tract during hepatitis

Abstract

Significant percentages of patients suffering from non-A non-B hepatitis (43%) and B hepatitis (35%) were found to release an Ig-binding factor in their stools. This factor, which we called "protein F" was less frequently observed (20%) in patients suffering from other liver disorders, and was found in only 6.7% of healthy subjects (p less than 10(-7), less than 10(-4), and less than 0.03, respectively). The specificity of the detection test (a nonimmune ELISA-like assay) was confirmed by inhibition experiments. Binding was located on the F(ab) fragment of Ig, irrespectively of their isotype. Protein F was inactivated by pepsin, neuraminidase, and high concentrations of subtilisin, whereas it was resistant to trypsin and chymotrypsin. Molecular sieving by HPLC indicated an apparent molecular mass of 175 kDa. In preparative SDS-PAGE, the molecular mass was 85 kDa in favor of a dimer disrupted under dissociating conditions. Preparative IEF showed the isoelectric charge to lie between 3.9 and 4.1. Analysis of liver extracts from two patients suffering fron non-A non-B hepatitis, and from a transplant donor, revealed the presence of the factor in the three cases.
No file

Dates and versions

pasteur-03236337 , version 1 (26-05-2021)

Identifiers

  • HAL Id : pasteur-03236337 , version 1
  • PUBMED : 2380553

Cite

Jean-Pierre Bouvet, René Pirès, Françoise Lunel-Fabiani, Bernadette Crescenzo-Chaigne, Patrick Maillard, et al.. Protein F. A novel F(ab)-binding factor, present in normal liver, and largely released in the digestive tract during hepatitis. Journal of Immunology, 1990, 145 (4), pp.1176-80. ⟨pasteur-03236337⟩

Collections

PASTEUR
10 View
1 Download

Altmetric

Share

Gmail Facebook X LinkedIn More