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Journal Articles Frontiers in Immunology Year : 2020

Systems Biology Methods Applied to Blood and Tissue for a Comprehensive Analysis of Immune Response to Hepatitis B Vaccine in Adults

Natallia Varankovich
  • Function : Author
Daniel He
  • Function : Author
Erin Gill
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Brian Aevermann
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Sibyl Drissler
  • Function : Author
Sarah Mccann
  • Function : Author
Kim Marty
  • Function : Author
Gordean Bjornson
  • Function : Author
Rachel Edgar
  • Function : Author
David Tse Shen Lin
  • Function : Author
Nicole Gladish
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Julia Maclsaac
  • Function : Author
Nelly Amenyogbe
  • Function : Author
Queenie Chan
  • Function : Author
Khoa Le
  • Function : Author
Colin Havenar-Daughton
  • Function : Author
Manraj Heran
  • Function : Author
Bippan Sangha
  • Function : Author
Mel Krajden
  • Function : Author
Shane Crotty
  • Function : Author
Devin Sok
  • Function : Author
Bryan Briney
  • Function : Author
Dennis Burton
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Leonard Foster
  • Function : Author
William Mohn
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Michael Kobor
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Scott Tebbutt
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Ryan Brinkman
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Richard Scheuermann
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Robert Hancock
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Abstract

Conventional vaccine design has been based on trial-and-error approaches, which have been generally successful. However, there have been some major failures in vaccine development and we still do not have highly effective licensed vaccines for tuberculosis, HIV, respiratory syncytial virus, and other major infections of global significance. Approaches at rational vaccine design have been limited by our understanding of the immune response to vaccination at the molecular level. Tools now exist to undertake in-depth analysis using systems biology approaches, but to be fully realized, studies are required in humans with intensive blood and tissue sampling. Methods that support this intensive sampling need to be developed and validated as feasible. To this end, we describe here a detailed approach that was applied in a study of 15 healthy adults, who were immunized with hepatitis B vaccine. Sampling included ~350 mL of blood, 12 microbiome samples, and lymph node fine needle aspirates obtained over a ~7-month period, enabling comprehensive analysis of the immune response at the molecular level, including single cell and tissue sample analysis. Samples were collected for analysis of immune phenotyping, whole blood and single cell gene expression, proteomics, lipidomics, epigenetics, whole blood response to key immune stimuli, cytokine responses, in vitro T cell responses, antibody repertoire analysis and the microbiome. Data integration was undertaken using different approaches-NetworkAnalyst and DIABLO. Our results demonstrate that such intensive sampling studies are feasible in healthy adults, and data integration tools exist to analyze the vast amount of data generated from a multi-omics systems biology approach. This will provide the basis for a better understanding of vaccine-induced immunity and accelerate future rational vaccine design.
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pasteur-03155985 , version 1 (02-03-2021)

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Rym Ben-Othman, Bing Cai, Aaron Liu, Natallia Varankovich, Daniel He, et al.. Systems Biology Methods Applied to Blood and Tissue for a Comprehensive Analysis of Immune Response to Hepatitis B Vaccine in Adults. Frontiers in Immunology, 2020, 11, pp.580373. ⟨10.3389/fimmu.2020.580373⟩. ⟨pasteur-03155985⟩

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