Fluid migration during bleb formation in cells in vivo - Institut Pasteur Access content directly
Journal Articles PLoS ONE Year : 2019

Fluid migration during bleb formation in cells in vivo

Abstract

Blebs are cellular protrusions observed in migrating cells and in cells undergoing spreading, cytokinesis, and apoptosis. Here we investigate the flow of cytoplasm during bleb formation and the concurrent changes in cell volume using zebrafish primordial germ cells (PGCs) as an in vivo model. We show that bleb inflation occurs concomitantly with cytoplasmic inflow into it and that during this process the total cell volume does not change. We thus show that bleb formation in primordial germ cells results primarily from redistribution of material within the cell rather than being driven by flow of water from an external source.
Fichier principal
Vignette du fichier
pone.0212699.pdf (2.83 Mo) Télécharger le fichier

Dates and versions

pasteur-03152920 , version 1 (25-02-2021)

Licence

Identifiers

Cite

Mohammad Goudarzi, Aleix Boquet-Pujadas, Jean-Christophe Olivo-Marin, Erez Raz. Fluid migration during bleb formation in cells in vivo. PLoS ONE, 2019, 14 (2), pp.e0212699. ⟨10.1371/journal.pone.0212699⟩. ⟨pasteur-03152920⟩
46 View
62 Download

Altmetric

Share

Gmail Mastodon Facebook X LinkedIn More