cDNA Cloning and Sequencing Reveal the Major Horse Allergen Equ c1 to Be a Glycoprotein Member of the Lipocalin Superfamily - Institut Pasteur Access content directly
Journal Articles Journal of Biological Chemistry Year : 1996

cDNA Cloning and Sequencing Reveal the Major Horse Allergen Equ c1 to Be a Glycoprotein Member of the Lipocalin Superfamily

Isabelle Rosinski-Chupin
Jacques Rabillon
  • Function : Author
Bernard David
  • Function : Author

Abstract

The gene encoding the major horse allergen, designated Equus caballus allergen 1 (Equ c1), was cloned from total cDNA of sublingual salivary glands by reverse transcription-polymerase chain reaction using synthetic degenerate oligonucleotides deduced from N-terminal and internal peptide sequences of the glycosylated hair dandruff protein. A recombinant form of the protein, with a polyhistidine tail, was expressed in Escherichia coli and purified by immobilized metal affinity chromatography. The recombinant protein is able to induce a passive cutaneous anaphylaxis reaction in rat, and it behaves similarly to the native Equ c1 in several immunological tests with allergic patients' IgE antibodies, mouse monoclonal antibodies, or rabbit polyclonal IgG antibodies. Amino acid sequence identity of 49-51% with rodent urinary proteins from mice and rats suggests that Equ c1 is a new member of the lipocalin superfamily of hydrophobic ligand-binding proteins that includes several other major allergens. An RNA blot analysis demonstrates the expression of mRNA Equ c1 in liver and in sublingual and submaxillary salivary glands.
Fichier principal
Vignette du fichier
1-s2.0-S0021925819788036-main.pdf (626.36 Ko) Télécharger le fichier

Dates and versions

pasteur-03136622 , version 1 (09-02-2021)

Licence

Identifiers

Cite

Christophe Gregoire, Isabelle Rosinski-Chupin, Jacques Rabillon, Pedro Alzari, Bernard David, et al.. cDNA Cloning and Sequencing Reveal the Major Horse Allergen Equ c1 to Be a Glycoprotein Member of the Lipocalin Superfamily. Journal of Biological Chemistry, 1996, 271 (51), pp.32951-32959. ⟨10.1074/jbc.271.51.32951⟩. ⟨pasteur-03136622⟩

Collections

PASTEUR
16 View
38 Download

Altmetric

Share

Gmail Mastodon Facebook X LinkedIn More