Diagnostic Assays for Crimean-Congo Hemorrhagic Fever - Institut Pasteur Access content directly
Journal Articles Emerging Infectious Diseases Year : 2012

Diagnostic Assays for Crimean-Congo Hemorrhagic Fever


Crimean-Congo hemorrhagic fever (CCHF) is a highly contagious viral tick-borne disease with case-fatality rates as high as 50%. We describe a collaborative evaluation of the characteristics, performance, and on-site applicability of serologic and molecular assays for diagnosis of CCHF. We evaluated ELISA, immunofluorescence, quantitative reverse transcription PCR, and low-density macroarray assays for detection of CCHF virus using precharacterized archived patient serum samples. Compared with results of local, in-house methods, test sensitivities were 87.8%-93.9% for IgM serology, 80.4%-86.1% for IgG serology, and 79.6%-83.3% for genome detection. Specificity was excellent for all assays; molecular test results were influenced by patient country of origin. Our findings demonstrate that well-characterized, reliable tools are available for CCHF diagnosis and surveillance. The on-site use of such assays by health laboratories would greatly diminish the time, costs, and risks posed by the handling, packaging, and shipping of highly infectious biologic material.


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pasteur-03112961 , version 1 (18-01-2021)





Jessica Vanhomwegen, Maria João Alves, Tatjana Avšič Županc, Silvia Bino, Sadegh Chinikar, et al.. Diagnostic Assays for Crimean-Congo Hemorrhagic Fever. Emerging Infectious Diseases, 2012, 18 (12), pp.1958-1965. ⟨10.3201/eid1812.120710⟩. ⟨pasteur-03112961⟩
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