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Book Sections Year : 2020

Isolation, Purification, and Characterization of Leptophages

Abstract

To date, only three bacteriophages of leptospires-leptophages-are known. Nonetheless, numerous prophages have been found in the genus, especially in the genomes of pathogenic species. Thus, some laboratories attempt to isolate leptophage particles from environmental samples or following mitomycin C induction of bacterial cultures. Here, we propose multiple procedures to isolate, purify, and characterize bacteriophages, based on protocols used for LE3 and LE4 characterization.
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pasteur-02951927 , version 1 (29-09-2020)

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Attribution - NonCommercial

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Olivier Schiettekatte, Pascale Bourhy. Isolation, Purification, and Characterization of Leptophages. Leptospira spp., 2134, Humana, pp.67-75, 2020, Methods in Molecular Biology, 978-1-0716-0459-5. ⟨10.1007/978-1-0716-0459-5_7⟩. ⟨pasteur-02951927⟩

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