Longitudinal dynamics of the human B cell response to the yellow fever 17D vaccine - Institut Pasteur Access content directly
Journal Articles Proceedings of the National Academy of Sciences of the United States of America Year : 2020

Longitudinal dynamics of the human B cell response to the yellow fever 17D vaccine

Anna Wec
  • Function : Author
Yasmina Abdiche
  • Function : Author
Laila Shehata
  • Function : Author
Crystal Moyer
  • Function : Author
Zachary Bornholdt
  • Function : Author
Asparouh Lilov
  • Function : Author
Juergen Nett
  • Function : Author
Rohit Jangra
  • Function : Author
Michael Brown
  • Function : Author
Clas Ahlm
  • Function : Author
  • PersonId : 908506
Mattias Forsell
  • Function : Author
Felix Rey
  • Function : Author
  • PersonId : 1058991
Laura Walker
  • Function : Author

Abstract

A comprehensive understanding of the development and evolution of human B cell responses induced by pathogen exposure will facilitate the design of next-generation vaccines. Here, we utilized a high-throughput single B cell cloning technology to longitudinally track the human B cell response to the yellow fever virus 17D (YFV-17D) vaccine. The early memory B cell (MBC) response was mediated by both classical immunoglobulin M (IgM) (IgM+CD27+) and switched immunoglobulin (swIg+) MBC populations; however, classical IgM MBCs waned rapidly, whereas swIg+ and atypical IgM+ and IgD+ MBCs were stable over time. Affinity maturation continued for 6 to 9 mo following vaccination, providing evidence for the persistence of germinal center activity long after the period of active viral replication in peripheral blood. Finally, a substantial fraction of the neutralizing antibody response was mediated by public clones that recognize a fusion loop-proximal antigenic site within domain II of the viral envelope glycoprotein. Overall, our findings provide a framework for understanding the dynamics and complexity of human B cell responses elicited by infection and vaccination.
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pasteur-02876540 , version 1 (21-06-2020)

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Attribution - NonCommercial - NoDerivatives

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Anna Wec, Denise Haslwanter, Yasmina Abdiche, Laila Shehata, Nuria Pedreño-Lopez, et al.. Longitudinal dynamics of the human B cell response to the yellow fever 17D vaccine. Proceedings of the National Academy of Sciences of the United States of America, 2020, 117 (12), pp.6675-6685. ⟨10.1073/pnas.1921388117⟩. ⟨pasteur-02876540⟩
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