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Journal Articles Genome Biology Year : 2020

instaGRAAL: chromosome-level quality scaffolding of genomes using a proximity ligation-based scaffolder

Abstract

Hi-C exploits contact frequencies between pairs of loci to bridge and order contigs during genome assembly, resulting in chromosome-level assemblies. Because few robust programs are available for this type of data, we developed instaGRAAL, a complete overhaul of the GRAAL program, which has adapted the latter to allow efficient assembly of large genomes. instaGRAAL features a number of improvements over GRAAL, including a modular correction approach that optionally integrates independent data. We validate the program using data for two brown algae, and human, to generate near-complete assemblies with minimal human intervention.
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pasteur-02873422 , version 1 (18-06-2020)

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Lyam Baudry, Nadège Guiglielmoni, Hervé Marie-Nelly, Alexandre Cormier, Komlan Avia, et al.. instaGRAAL: chromosome-level quality scaffolding of genomes using a proximity ligation-based scaffolder. Genome Biology, 2020, 21 (1), ⟨10.1186/s13059-020-02041-z⟩. ⟨pasteur-02873422⟩
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