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Preprints, Working Papers, ... Year : 2020

Argonaute catalytic activity is required for maternal mRNA clearance in embryos

Abstract

Argonaute proteins and their interacting small RNAs play a key role in regulating complementary mRNA targets during animal development. Here, we investigate a novel and essential function of the catalytically active Argonaute protein CSR-1 in maternal mRNA degradation during early embryogenesis in Caenorhabditis elegans. We show that CSR-1 interacts with endogenous small RNAs antisense to hundreds of cleared maternal mRNAs in embryos, and preferentially cleaves mRNAs no longer engaged in translation. The depletion of CSR-1 during maternal to zygotic transition leads to embryonic lethality in a catalytic-dependent manner and impairs the degradations of its embryonic mRNA targets. Given the conservation of Argonaute catalytic activity, we propose that a similar mechanism operate to clear maternal mRNAs during maternal to zygotic transition across species.
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pasteur-02626442 , version 1 (26-05-2020)

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Piergiuseppe Quarato, Meetali Singh, Eric Cornes, Blaise Maxime Li, Loan Bourdon, et al.. Argonaute catalytic activity is required for maternal mRNA clearance in embryos. 2020. ⟨pasteur-02626442⟩
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