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Journal Articles European Journal of Immunology Year : 2019

Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)

Andrea Cossarizza (1) , Hyun‐dong Chang (2) , Andreas Radbruch (2) , Andreas Acs , Dieter Adam , Sabine Adam‐klages , William Agace , Nima Aghaeepour , Mübeccel Akdis (3) , Matthieu Allez (4, 5) , Larissa Nogueira Almeida , Giorgia Alvisi , Graham Anderson , Immanuel Andrä , Francesco Annunziato (6) , Achille Anselmo , Petra Bacher (7) , Cosima Baldari (8) , Sudipto Bari , Vincenzo Barnaba (9) , Joana Barros‐martins , Luca Battistini (10) , Wolfgang Bauer , Sabine Baumgart (11) , Nicole Baumgarth , Dirk Baumjohann , Bianka Baying , Mary Bebawy , Burkhard Becher , Wolfgang Beisker (12) , Vladimir Benes (13) , Rudi Beyaert (14) , Alfonso Blanco , Dominic Boardman , Christian Bogdan , Jessica Borger , Giovanna Borsellino (15) , Philip Boulais , Jolene Bradford , Dirk Brenner , Ryan Brinkman , Anna Brooks , Dirk Busch , Martin Büscher , Timothy Bushnell , Federica Calzetti , Garth Cameron , Ilenia Cammarata , Xuetao Cao (16) , Susanna Cardell , Stefano Casola (17) , Marco Cassatella , Andrea Cavani , Antonio Celada , Lucienne Chatenoud (18) , Pratip Chattopadhyay , Sue Chow , Eleni Christakou , Luka Čičin‐šain , Mario Clerici (19) , Federico Colombo , Laura Cook , Anne Cooke , Andrea Cooper , Alexandra Corbett (20) , Antonio Cosma (21) , Lorenzo Cosmi , Pierre Coulie (22) , Ana Cumano (23) , Ljiljana Cvetkovic , van Duc Dang , Chantip Dang‐heine , Martin Davey , Derek Davies , Sara de Biasi , Genny del Zotto , Gelo Victoriano Dela Cruz , Michael Delacher , Silvia Della Bella , Paolo Dellabona (24) , Günnur Deniz , Mark Dessing , James Di Santo (25) , Andreas Diefenbach (26) , Francesco Dieli (27) , Andreas Dolf , Thomas Dörner (28) , Regine Dress (29) , Diana Dudziak (30) , Michael Dustin (31) , Charles‐antoine Dutertre , Friederike Ebner , Sidonia Eckle , Matthias Edinger , Pascale Eede , Götz R.A. Ehrhardt , Marcus Eich , Pablo Engel , Britta Engelhardt (32) , Anna Erdei , Charlotte Esser (33) , Bart Everts , Maximilien Évrard (34) , Christine Falk , Todd Fehniger , Mar Felipo‐benavent , Helen Ferry , Markus Feuerer , Andrew Filby , Kata Filkor , Simon Fillatreau (35) , Marie Follo , Irmgard Förster , John Foster , Gemma Foulds , Britta Frehse , Paul Frenette , Stefan Frischbutter , Wolfgang Fritzsche , David Galbraith (36) , Anastasia Gangaev , Natalio Garbi , Brice Gaudilliere , Ricardo Gazzinelli , Jens Geginat (37) , Wilhelm Gerner , Nicholas Gherardin , Kamran Ghoreschi , Lara Gibellini , Florent Ginhoux (38) , Keisuke Goda , Dale Godfrey , Christoph Goettlinger , Jose González‐navajas , Carl Goodyear , Andrea Gori (39) , Jane Grogan , Daryl Grummitt , Andreas Grützkau (40) , Claudia Haftmann (41) , Jonas Hahn , Hamida Hammad (14) , Günter Hämmerling , Leo Hansmann , Göran K. Hansson (42) , Christopher Harpur , Susanne Hartmann , Andrea Hauser , Anja Hauser , David Haviland , David Hedley , Daniela Hernández , Guadalupe Herrera , Martin Herrmann (43) , Christoph Hess , Thomas Höfer (41) , Petra Hoffmann , Kristin Hogquist , Tristan Holland , Thomas Höllt (44) , Rikard Holmdahl (45) , Pleun Hombrink , Jessica Houston , Bimba Hoyer , Bo Huang (46) , Fang‐ping Huang , Johanna Huber , Jochen Huehn (47) , Michael Hundemer (48) , Christopher Hunter , William Hwang , Anna Iannone , Florian Ingelfinger , Sabine Ivison , Hans‐martin Jäck , Peter Jani , Beatriz Jávega , Stipan Jonjic (49) , Toralf Kaiser , Tomas Kalina , Thomas Kamradt , Stefan Kaufmann (50) , Baerbel Keller (51) , Steven Ketelaars , Ahad Khalilnezhad , Srijit Khan , Jan Kisielow , Paul Klenerman (52) , Jasmin Knopf , Hui‐fern Koay , Katja Kobow , Jay Kolls , Wan Ting Kong , Manfred Kopf (53) , Thomas Korn , Katharina Kriegsmann , Hendy Kristyanto , Thomas Kroneis , Andreas Krueger , Jenny Kühne , Christian Kukat , Désirée Kunkel (54) , Heike Kunze‐schumacher , Tomohiro Kurosaki (55) , Christian Kurts (56) , Pia Kvistborg , Immanuel Kwok , Jonathan Landry , Olivier Lantz (57) , Paola Lanuti , Francesca Larosa , Agnès Lehuen (58) , Salomé Leibundgut‐landmann , Michael Leipold , Leslie Y.T. Leung , Megan Levings (59) , Andreia Lino , Francesco Liotta , Virginia Litwin , Yanling Liu (60) , Hans‐gustaf Ljunggren , Michael Lohoff (61) , Giovanna Lombardi (62) , Lilly Lopez , Miguel López‐botet , Amy Lovett‐racke , Erik Lubberts (63) , Hervé Luche (64) , Burkhard Ludewig (65) , Enrico Lugli (66) , Sebastian Lunemann (67) , Holden Maecker , Laura Maggi (68) , Orla Maguire , Florian Mair , Kerstin Mair , Alberto Mantovani (69) , Rudolf Manz , Aaron Marshall , Alicia Martínez‐romero , Gloria Martrus (67) , Ivana Marventano (70) , Wlodzimierz Maslinski , Giuseppe Matarese , Anna Vittoria Mattioli , Christian Maueröder , Alessio Mazzoni , James Mccluskey (20) , Mairi Mcgrath (71) , Helen Mcguire , Iain Mcinnes , Henrik Mei (72) , Fritz Melchers , Susanne Melzer , Dirk Mielenz , Stephen Miller , Kingston H.G. Mills (73) , Hans Minderman , Jenny Mjösberg (74) , Jonni Moore , Barry Moran , Lorenzo Moretta (75) , Tim Mosmann , Susann Müller (76) , Gabriele Multhoff (77) , Luis Enrique Muñoz , Christian Münz (78) , Toshinori Nakayama , Milena Nasi , Katrin Neumann (79) , Lai Guan Ng , Antonia Niedobitek , Sussan Nourshargh (80) , Gabriel Nùñez (81) , José‐enrique O'Connor , Aaron Ochel , Anna Oja , Diana Ordonez , Alberto Orfao (82) , Eva Orlowski‐oliver , Wenjun Ouyang , Annette Oxenius , Raghavendra Palankar , Isabel Panse (41) , Kovit Pattanapanyasat , Malte Paulsen , Dinko Pavlinic , Livius Penter , Pärt Peterson (83) , Christian Peth , Jordi Petriz , Federica Piancone , Winfried Pickl , Silvia Piconese (9) , Marcello Pinti (1) , A. Graham Pockley , Malgorzata Justyna Podolska , Zhiyong Poon , Katharina Pracht , Immo Prinz (84) , Carlo Pucillo , Sally Quataert , Linda Quatrini (85) , Kylie M Quinn (86) , Helena Radbruch , Tim Radstake , Susann Rahmig , Hans‐peter Rahn , Bartek Rajwa (87) , Gevitha Ravichandran , Yotam Raz , Jonathan Rebhahn , Diether Recktenwald , Dorothea Reimer , Caetano Reis E Sousa , Ester B.M. Remmerswaal , Lisa Richter , Laura Rico , Andy Riddell , Aja Rieger , J. Paul Robinson (87) , Chiara Romagnani (2) , Anna Rubartelli , Jürgen Ruland , Armin Saalmüller , Yvan Saeys (88) , Takashi Saito (89) , Shimon Sakaguchi (90) , Francisco Sala‐de‐oyanguren , Yvonne Samstag , Sharon Sanderson , Inga Sandrock , Angela Santoni (91) , Ramon Bellmàs Sanz , Marina Saresella (70) , Catherine Sautes‐fridman (92) , Birgit Sawitzki (93) , Linda Schadt , Alexander Scheffold , Hans Scherer , Matthias Schiemann , Frank Schildberg , Esther Schimisky , Andreas Schlitzer (38) , Josephine Schlosser , Stephan Schmid , Steffen Schmitt (94) , Kilian Schober , Daniel Schraivogel , Wolfgang Schuh (95) , Thomas Schüler (96) , Reiner Schulte , Axel Ronald Schulz , Sebastian Schulz (97) , Cristiano Scottá , Daniel Scott-Algara (98) , David Sester , T. Vincent Shankey , Bruno Silva‐santos , Anna Katharina Simon , Katarzyna Sitnik , Silvano Sozzani (99) , Daniel Speiser (100) , Josef Spidlen (101) , Anders Stahlberg , Alan Stall , Natalie Stanley , Regina Stark , Christina Stehle , Tobit Steinmetz , Hannes Stockinger (102) , Yousuke Takahama , Kiyoshi Takeda , Leonard Tan , Attila Tarnok (103) , Gisa Tiegs (104) , Gergely Toldi , Julia Tornack , Elisabetta Traggiai , Mohamed Trebak (105) , Timothy I.M. Tree , Joe Trotter , John Trowsdale (106) , Maria Tsoumakidou , Henning Ulrich , Sophia Urbanczyk , Willem Veen (78) , Maries Broek , Edwin Pol , Sofie van Gassen (107) , Gert van Isterdael (108) , René A.W. Lier , Marc Veldhoen (109) , Salvador Vento‐asturias , Paulo Vieira (23) , David Voehringer (110) , Hans‐dieter Volk , Anouk Borstel , Konrad Volkmann , Ari Waisman (111) , Rachael Walker , Paul Wallace (112) , Sa Wang , Xin Wang (113) , Michael Ward (114) , Kirsten Ward‐hartstonge , Klaus Warnatz (51) , Gary Warnes (115) , Sarah Warth , Claudia Waskow (116) , James Watson , Carsten Watzl , Leonie Wegener , Thomas Weisenburger , Annika Wiedemann , Jürgen Wienands , Anneke Wilharm , Robert John Wilkinson , Gerald Willimsky , James Wing , Rieke Winkelmann , Thomas Winkler , Oliver Wirz (78) , Alicia Wong , Peter Wurst , Jennie Yang , Juhao Yang , Maria Yazdanbakhsh , Liping Yu (117) , Alice Yue , Hanlin Zhang (118) , Yi Zhao (119) , Susanne Maria Ziegler (67) , Christina Zielinski , Jakob Zimmermann (120) , Arturo Zychlinsky (121, 98)
1 UNIMORE - Università degli Studi di Modena e Reggio Emilia = University of Modena and Reggio Emilia
2 DRFZ - Deutsches Rheuma-ForschungsZentrum
3 SIAF - Swiss Institute of Allergy and Asthma Research
4 IRSL - Institut de Recherche Saint-Louis - Hématologie Immunologie Oncologie (Département de recherche de l’UFR de médecine ; ex- Institut Universitaire Hématologie-IUH)
5 EMily (UMR_S_1160 / U1160) - Ecotaxie, microenvironnement et développement lymphocytaire
6 Department of Internal Medicine
7 Institute of Clinical Molecular Biology
8 Department of Life Sciences [Siena, Italy]
9 Institut Pasteur, Fondation Cenci Bolognetti - Istituto Pasteur Italia, Fondazione Cenci Bolognetti
10 Dulbecco Telethon Institute/Department of Biology
11 Caprotec Bioanalytics GmbH
12 IOTA ES - International Occultation Timing Association European Section
13 EMBL - European Molecular Biology Laboratory [Heidelberg]
14 IRC - VIB-UGent Center for Inflammation Research [Gand, Belgique]
15 IRCCS - Fondazione Santa Lucia
16 Department of Immunology
17 FIRC Institute of Molecular Oncology Foundation
18 INEM - UM 111 (UMR 8253 / U1151) - Institut Necker Enfants-Malades
19 DEPT - Department of Physiopatology and Transplantation, University of Milan
20 Monash University [Clayton]
21 IMETI - Institut des Maladies Emergentes et des Thérapies Innovantes
22 Institute of Cellular Pathology
23 Lymphopoïèse (UMR_1223 / U1223 / U-Pasteur_4) - Lymphopoïèse
24 Experimental Immunology Unit, Dept. of Oncology
25 Immunité Innée - Innate Immunity
26 Charité - UniversitätsMedizin = Charité - University Hospital [Berlin]
27 Institute for Medical Immunology IMI - Department of Biopharmacy [Bruxelles, Belgium]
28 Charité Hospital
29 A*STAR - Agency for science, technology and research [Singapore]
30 Laboratory of Molecular Immunology and the Howard Hughes Institute
31 Kennedy Institute of Rheumatology [Oxford, UK]
32 Theodor Kocher Institute
33 IUF - Leibniz Research Institute for Environmental Medicine [Düsseldorf, Germany]
34 UL2 - Université Lumière - Lyon 2
35 INEM - UM 111 (UMR 8253 / U1151) - Institut Necker Enfants-Malades
36 Edin. - University of Edinburgh
37 Integrative Biology Program [Milano]
38 SIgN - Singapore Immunology Network
39 UB - Universitat de Barcelona
40 Rheumatologie
41 Cell Biology
42 Department of medicine [Stockholm]
43 Department for Internal Medicine 3, Institute for Clinical Immunology
44 TU Delft - Delft University of Technology
45 Medical Inflammation Research
46 Department of Photonics Engineering [Lyngby]
47 Dpt of Experimental Immunology [Braunschweig]
48 Department of Internal Medicine V
49 Department of Histology and Embryology
50 Universität Heidelberg [Heidelberg] = Heidelberg University
51 Uniklinik - Universitäts Klinikum Freiburg = University Medical Center Freiburg
52 Nuffield Dept of Clinical Medicine
53 Institute of Integrative Biology, Molecular Biomedicine
54 BIH - Berlin Institute of Health
55 Laboratory for Lymphocyte Differentiation
56 Institutes of Molecular Medicine and Experimental Immunology
57 U932 - Immunité et cancer
58 IC UM3 (UMR 8104 / U1016) - Institut Cochin
59 Child and Family Research Institute - Department of Surgery [Vancouver, BC, Canada]
60 College of Food Science and Technology [Shangai]
61 Institute for Medical Microbiology and Hygiene
62 King‘s College London
63 Erasmus MC - Erasmus University Medical Center [Rotterdam]
64 CIPHE - Centre d'Immunophénomique
65 Brustzentrum Kantonsspital St. Gallen
66 Immunotechnology Section, Vaccine Research Center
67 Heinrich Pette Institute [Hamburg]
68 UniFI - Università degli Studi di Firenze = University of Florence = Université de Florence
69 Department of Immunology and Cell Biology
70 Laboratory of Molecular Medicine and Biotechnology
71 Institute of Translational Medicine
72 Klinik für Dermatologie, Venerologie und Allergologie
73 School of Biochemistry and Immunology
74 Department of Medicine Huddinge
75 UniGe - Università degli studi di Genova = University of Genoa
76 Department of Environmental Microbiology
77 Department of Radiation Oncology [Munich]
78 UZH - Universität Zürich [Zürich] = University of Zurich
79 Centre de Recherche Publique- Santé
80 William Harvey Research Institute, Barts and the London Medical School
81 University of Michigan [Ann Arbor]
82 CSIC - Centro de Investigacion del Cancer
83 Molecular Pathology [Tartu, Estonia]
84 MHH - Medizinische Hochschule Hannover = Hannover Medical School
85 CIML - Centre d'Immunologie de Marseille - Luminy
86 Monash Biomedicine Discovery Institute
87 Cytometry Laboratories and School of Veterinary Medicine
88 Data Mining and Modelling for Biomedicine [Ghent, Belgium]
89 Laboratory for Cell Signaling, RIKEN Research Center for Allergy and Immunology
90 Osaka University [Osaka]
91 UNIROMA - Università degli Studi di Roma "La Sapienza" = Sapienza University [Rome]
92 CRC (UMR_S_1138 / U1138) - Centre de Recherche des Cordeliers
93 Institute of Medical Immunology [Berlin, Germany]
94 FACS and Array Core Facility
95 FAU - Friedrich-Alexander Universität Erlangen-Nürnberg = University of Erlangen-Nuremberg
96 OVGU - Otto-von-Guericke-Universität Magdeburg = Otto-von-Guericke University [Magdeburg]
97 SUPA School of Physics and Astronomy [University of St Andrews]
98 Biologie Cellulaire des Lymphocytes - Lymphocyte Cell Biology
99 GPI - General Pathology and Immunology
100 UNIL - Université de Lausanne = University of Lausanne
101 Terry Fox Laboratory
102 Department of Molecular Immunology
103 Dept. Pediatric Cardiology
104 UKE - Universitaetsklinikum Hamburg-Eppendorf = University Medical Center Hamburg-Eppendorf [Hamburg]
105 Center for Cardiovascular Sciences
106 Dept Pathol, Div Immunol
107 Department of Information Technology [Gent]
108 Department of Plant Systems Biology, Department of Plant Biotechnology and Genetics
109 Division of Molecular Immunology
110 Institute for Immunology
111 Department of Internal Medicine
112 Department of Geological Sciences
113 CDC - Centers for Disease Control and Prevention [Atlanta]
114 University of Colorado [Colorado Springs]
115 FACS laboratory, Cancer Research, London
116 Regeneration in Hematopoiesis and Animal Models of Hematopoiesis
117 BDC - Barbara Davis Center for Childhood Diabetes
118 School of Computer and Electronic Information [Guangxi University]
119 School of Materials Science and Engineering
120 UNIBE - Universität Bern / University of Bern
121 MPIIB - Max Planck Institute for Infection Biology
Andreas Acs
  • Function : Author
Dieter Adam
  • Function : Author
Sabine Adam‐klages
  • Function : Author
William Agace
Nima Aghaeepour
  • Function : Author
Larissa Nogueira Almeida
  • Function : Author
Giorgia Alvisi
  • Function : Author
Graham Anderson
  • Function : Author
Immanuel Andrä
  • Function : Author
Francesco Annunziato
  • Function : Author
  • PersonId : 934993
Achille Anselmo
  • Function : Author
Sudipto Bari
  • Function : Author
Joana Barros‐martins
  • Function : Author
Wolfgang Bauer
  • Function : Author
Nicole Baumgarth
  • Function : Author
Dirk Baumjohann
  • Function : Author
Bianka Baying
  • Function : Author
Mary Bebawy
  • Function : Author
Burkhard Becher
Alfonso Blanco
  • Function : Author
Dominic Boardman
  • Function : Author
Christian Bogdan
  • Function : Author
Jessica Borger
  • Function : Author
Philip Boulais
  • Function : Author
Jolene Bradford
  • Function : Author
Dirk Brenner
  • Function : Author
Ryan Brinkman
  • Function : Author
Anna Brooks
  • Function : Author
Dirk Busch
  • Function : Author
Martin Büscher
  • Function : Author
Timothy Bushnell
  • Function : Author
Federica Calzetti
  • Function : Author
Garth Cameron
  • Function : Author
Ilenia Cammarata
  • Function : Author
Xuetao Cao
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  • PersonId : 924466
Susanna Cardell
  • Function : Author
Marco Cassatella
  • Function : Author
Andrea Cavani
  • Function : Author
Antonio Celada
  • Function : Author
Pratip Chattopadhyay
  • Function : Author
Sue Chow
  • Function : Author
Eleni Christakou
  • Function : Author
Luka Čičin‐šain
  • Function : Author
Federico Colombo
Laura Cook
  • Function : Author
Anne Cooke
  • Function : Author
Andrea Cooper
  • Function : Author
Alexandra Corbett
Lorenzo Cosmi
  • Function : Author
Ana Cumano
  • Function : Author
Ljiljana Cvetkovic
  • Function : Author
van Duc Dang
  • Function : Author
Chantip Dang‐heine
  • Function : Author
Martin Davey
  • Function : Author
Derek Davies
  • Function : Author
Sara de Biasi
  • Function : Author
Genny del Zotto
Gelo Victoriano Dela Cruz
  • Function : Author
Michael Delacher
  • Function : Author
Silvia Della Bella
  • Function : Author
Günnur Deniz
  • Function : Author
Mark Dessing
  • Function : Author
James Di Santo
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  • PersonId : 915884
Andreas Dolf
  • Function : Author
Thomas Dörner
Michael Dustin
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  • PersonId : 1045767
Charles‐antoine Dutertre
  • Function : Author
Friederike Ebner
  • Function : Author
Sidonia Eckle
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Matthias Edinger
  • Function : Author
Pascale Eede
  • Function : Author
Götz R.A. Ehrhardt
  • Function : Author
Marcus Eich
  • Function : Author
Pablo Engel
  • Function : Author
Anna Erdei
  • Function : Author
Bart Everts
  • Function : Author
Christine Falk
  • Function : Author
Todd Fehniger
  • Function : Author
Mar Felipo‐benavent
  • Function : Author
Helen Ferry
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Markus Feuerer
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Andrew Filby
  • Function : Author
Kata Filkor
  • Function : Author
Simon Fillatreau
Marie Follo
  • Function : Author
Irmgard Förster
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John Foster
  • Function : Author
Gemma Foulds
  • Function : Author
Britta Frehse
  • Function : Author
Paul Frenette
  • Function : Author
Stefan Frischbutter
  • Function : Author
Wolfgang Fritzsche
  • Function : Author
Anastasia Gangaev
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Natalio Garbi
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Brice Gaudilliere
Ricardo Gazzinelli
  • Function : Author
Wilhelm Gerner
  • Function : Author
Nicholas Gherardin
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Kamran Ghoreschi
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Lara Gibellini
  • Function : Author
Florent Ginhoux
Keisuke Goda
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Dale Godfrey
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Christoph Goettlinger
  • Function : Author
Jose González‐navajas
  • Function : Author
Carl Goodyear
  • Function : Author
Jane Grogan
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Daryl Grummitt
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Andreas Grützkau
  • Function : Author
Claudia Haftmann
  • Function : Author
Jonas Hahn
  • Function : Author
Günter Hämmerling
  • Function : Author
Leo Hansmann
  • Function : Author
Christopher Harpur
  • Function : Author
Susanne Hartmann
  • Function : Author
Andrea Hauser
  • Function : Author
Anja Hauser
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David Haviland
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David Hedley
  • Function : Author
Daniela Hernández
  • Function : Author
Guadalupe Herrera
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Christoph Hess
  • Function : Author
Thomas Höfer
  • Function : Author
Petra Hoffmann
  • Function : Author
Kristin Hogquist
  • Function : Author
Tristan Holland
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Rikard Holmdahl
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  • PersonId : 933001
Pleun Hombrink
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Jessica Houston
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Bimba Hoyer
  • Function : Author
Fang‐ping Huang
  • Function : Author
Johanna Huber
  • Function : Author
Christopher Hunter
  • Function : Author
William Hwang
  • Function : Author
Anna Iannone
  • Function : Author
Florian Ingelfinger
  • Function : Author
Sabine Ivison
  • Function : Author
Hans‐martin Jäck
  • Function : Author
Peter Jani
  • Function : Author
Beatriz Jávega
  • Function : Author
Toralf Kaiser
  • Function : Author
Tomas Kalina
  • Function : Author
Thomas Kamradt
  • Function : Author
Steven Ketelaars
  • Function : Author
Ahad Khalilnezhad
  • Function : Author
Srijit Khan
  • Function : Author
Jan Kisielow
  • Function : Author
Paul Klenerman
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  • PersonId : 914387
Jasmin Knopf
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Hui‐fern Koay
  • Function : Author
Katja Kobow
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Jay Kolls
  • Function : Author
Wan Ting Kong
  • Function : Author
Thomas Korn
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Katharina Kriegsmann
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Hendy Kristyanto
  • Function : Author
Thomas Kroneis
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Andreas Krueger
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Jenny Kühne
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Christian Kukat
  • Function : Author
Heike Kunze‐schumacher
  • Function : Author
Tomohiro Kurosaki
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  • PersonId : 853467
Pia Kvistborg
  • Function : Author
Immanuel Kwok
  • Function : Author
Jonathan Landry
  • Function : Author
Olivier Lantz
Paola Lanuti
  • Function : Author
Francesca Larosa
  • Function : Author
Agnès Lehuen
Salomé Leibundgut‐landmann
  • Function : Author
Michael Leipold
  • Function : Author
Leslie Y.T. Leung
  • Function : Author
Andreia Lino
  • Function : Author
Francesco Liotta
  • Function : Author
Virginia Litwin
  • Function : Author
Hans‐gustaf Ljunggren
  • Function : Author
Lilly Lopez
  • Function : Author
Miguel López‐botet
  • Function : Author
Amy Lovett‐racke
  • Function : Author
Holden Maecker
  • Function : Author
Orla Maguire
  • Function : Author
Florian Mair
  • Function : Author
Kerstin Mair
  • Function : Author
Rudolf Manz
  • Function : Author
Aaron Marshall
  • Function : Author
Alicia Martínez‐romero
  • Function : Author
Wlodzimierz Maslinski
  • Function : Author
Giuseppe Matarese
  • Function : Author
Anna Vittoria Mattioli
  • Function : Author
Christian Maueröder
  • Function : Author
Alessio Mazzoni
  • Function : Author
James Mccluskey
Helen Mcguire
  • Function : Author
Iain Mcinnes
  • Function : Author
Fritz Melchers
  • Function : Author
Susanne Melzer
  • Function : Author
Dirk Mielenz
  • Function : Author
Stephen Miller
Hans Minderman
  • Function : Author
Jonni Moore
  • Function : Author
Barry Moran
  • Function : Author
Tim Mosmann
  • Function : Author
Susann Müller
  • Function : Author
  • PersonId : 871120
Luis Enrique Muñoz
  • Function : Author
Toshinori Nakayama
  • Function : Author
Milena Nasi
  • Function : Author
Lai Guan Ng
  • Function : Author
Antonia Niedobitek
  • Function : Author
José‐enrique O'Connor
  • Function : Author
Aaron Ochel
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Anna Oja
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Diana Ordonez
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Alberto Orfao
Eva Orlowski‐oliver
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Wenjun Ouyang
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Annette Oxenius
  • Function : Author
Raghavendra Palankar
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Isabel Panse
  • Function : Author
Kovit Pattanapanyasat
  • Function : Author
Malte Paulsen
  • Function : Author
Dinko Pavlinic
  • Function : Author
Livius Penter
  • Function : Author
Pärt Peterson
Christian Peth
  • Function : Author
Jordi Petriz
  • Function : Author
Federica Piancone
  • Function : Author
Winfried Pickl
  • Function : Author
A. Graham Pockley
  • Function : Author
Malgorzata Justyna Podolska
  • Function : Author
Zhiyong Poon
  • Function : Author
Katharina Pracht
  • Function : Author
Carlo Pucillo
  • Function : Author
Sally Quataert
  • Function : Author
Helena Radbruch
  • Function : Author
Tim Radstake
  • Function : Author
Susann Rahmig
  • Function : Author
Hans‐peter Rahn
  • Function : Author
Gevitha Ravichandran
  • Function : Author
Yotam Raz
  • Function : Author
Jonathan Rebhahn
  • Function : Author
Diether Recktenwald
  • Function : Author
Dorothea Reimer
  • Function : Author
Caetano Reis E Sousa
  • Function : Author
Ester B.M. Remmerswaal
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Abstract

These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human cells. The latest flow cytometry techniques and applications are also described, featuring examples of the data that can be generated and, importantly, how the data can be analysed. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid, all written and peer-reviewed by leading experts in the field, making this an essential research companion.

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Immunology
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pasteur-02573762 , version 1 (13-04-2024)

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Andrea Cossarizza, Hyun‐dong Chang, Andreas Radbruch, Andreas Acs, Dieter Adam, et al.. Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition). European Journal of Immunology, 2019, 49 (10), pp.1457-1973. ⟨10.1002/eji.201970107⟩. ⟨pasteur-02573762⟩
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