Saporin-conjugated tetramers identify efficacious anti-HIV CD8+ T-cell specificities - Institut Pasteur Access content directly
Journal Articles PLoS ONE Year : 2017

Saporin-conjugated tetramers identify efficacious anti-HIV CD8+ T-cell specificities

Abstract

Antigen-specific T-cells are highly variable, spanning potent antiviral efficacy and damaging auto-reactivity. In virus infections, identifying the most efficacious responses is critical to vaccine design. However, current methods depend on indirect measures or on ex vivo expanded CTL clones. We here describe a novel application of cytotoxic saporin-conjugated tetramers to kill antigen-specific T-cells without significant off-target effects. The relative efficacy of distinct antiviral CD8+ T-cell specificity can be directly assessed via antigen-specific CD8+ T-cell depletion. The utility of these reagents is demonstrated here in identifying the CD8+ T-cell specificity most effective in preventing HIV progression in HIV-infected HLA-B*27-positive immune controllers.
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pasteur-02549950 , version 1 (21-04-2020)

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Ellen Leitman, Christine Palmer, Søren Buus, Fabian Chen, Lynn Riddell, et al.. Saporin-conjugated tetramers identify efficacious anti-HIV CD8+ T-cell specificities. PLoS ONE, 2017, 12 (10), pp.e0184496. ⟨10.1371/journal.pone.0184496⟩. ⟨pasteur-02549950⟩

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