The Ancient Gamete Fusogen HAP2 Is a Eukaryotic Class II Fusion Protein - Institut Pasteur Access content directly
Journal Articles Cell Year : 2017

The Ancient Gamete Fusogen HAP2 Is a Eukaryotic Class II Fusion Protein

Abstract

Sexual reproduction is almost universal in eukaryotic life and involves the fusion of male and female haploid gametes into a diploid cell. The sperm-restricted single-pass transmembrane protein HAP2-GCS1 has been postulated to function in membrane merger. Its presence in the major eukaryotic taxa-animals, plants, and protists (including important human pathogens like Plasmodium)-suggests that many eukaryotic organisms share a common gamete fusion mechanism. Here, we report combined bioinformatic, biochemical, mutational, and X-ray crystallographic studies on the unicellular alga Chlamydomonas reinhardtii HAP2 that reveal homology to class II viral membrane fusion proteins. We further show that targeting the segment corresponding to the fusion loop by mutagenesis or by antibodies blocks gamete fusion. These results demonstrate that HAP2 is the gamete fusogen and suggest a mechanism of action akin to viral fusion, indicating a way to block Plasmodium transmission and highlighting the impact of virus-cell genetic exchanges on the evolution of eukaryotic life.
Fichier principal
Vignette du fichier
1-s2.0-S0092867417301095-main.pdf (6.15 Mo) Télécharger le fichier
1-s2.0-S0092867417301095-mmc1.pdf (57.72 Ko) Télécharger le fichier
Loading...

Dates and versions

pasteur-02546464 , version 1 (17-04-2020)

Licence

Identifiers

Cite

Juliette Fedry, Yanjie Liu, Gerard Pehau-Arnaudet, Jimin Pei, Wenhao Li, et al.. The Ancient Gamete Fusogen HAP2 Is a Eukaryotic Class II Fusion Protein. Cell, 2017, 168 (5), pp.904-915.e10. ⟨10.1016/j.cell.2017.01.024⟩. ⟨pasteur-02546464⟩
70 View
183 Download

Altmetric

Share

Gmail Mastodon Facebook X LinkedIn More