Subcutaneous vaccination with a live attenuated Yersinia pseudotuberculosis plague vaccine - Institut Pasteur Access content directly
Journal Articles Vaccine Year : 2020

Subcutaneous vaccination with a live attenuated Yersinia pseudotuberculosis plague vaccine

Anne Derbise
Chloé Guillas
  • Function : Author
Elisabeth Carniel
  • Function : Author
  • PersonId : 1039366
Javier Pizarro-Cerdá
Christian E. Demeure

Abstract

A single oral inoculation to mice of the live attenuated Yersinia pseudotuberculosis VTnF1 strain producing an F1 pseudocapsule protects against bubonic and pneumonic plague. However oral vaccination can fail in humans exposed to frequent intestinal infections. We evaluated in mice the efficacy of subcutaneous vaccine injection as an alternative way to induce protective immunity, while reducing the dose and avoiding strain release in nature. A single subcutaneous dose of up to 108 CFU induced dose-dependent antibody production. At the dose of 107 CFU, i.e. 10 times less than via the oral route, it caused a modest skin reaction and protected 100% against bubonic and 80% against pneumonic plague, caused by high doses of Yersinia pestis. Bacteria migrating to lymph nodes and spleen, but not feces, were rapidly eliminated. Thus, subcutaneous injection of VTnF1 would represent a good alternative when dissemination in nature and human intestinal responsiveness are limitations.
Fichier principal
Vignette du fichier
2020 Derbise JVAC.pdf (852.35 Ko) Télécharger le fichier
Origin : Publication funded by an institution
Loading...

Dates and versions

pasteur-02493687 , version 1 (28-02-2020)

Licence

Attribution - NonCommercial - NoDerivatives

Identifiers

Cite

Anne Derbise, Chloé Guillas, Christiane Gerke, Elisabeth Carniel, Javier Pizarro-Cerdá, et al.. Subcutaneous vaccination with a live attenuated Yersinia pseudotuberculosis plague vaccine. Vaccine, 2020, 38 (8), pp.1888-1892. ⟨10.1016/j.vaccine.2020.01.014⟩. ⟨pasteur-02493687⟩

Collections

PASTEUR ANR
219 View
150 Download

Altmetric

Share

Gmail Facebook X LinkedIn More