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Journal Articles Nature Methods Year : 2019

Best practices and benchmarks for intact protein analysis for top-down mass spectrometry

Jeremy Wolff
  • Function : Author
Paul Danis
  • Function : Author
Yury Tsybin
  • Function : Author

Abstract

One gene can give rise to many functionally distinct proteoforms, each of which has a characteristic molecular mass. Top-down mass spectrometry enables the analysis of intact proteins and proteoforms. Here members of the Consortium for Top-Down Proteomics provide a decision tree that guides researchers to robust protocols for mass analysis of intact proteins (antibodies, membrane proteins and others) from mixtures of varying complexity. We also present cross-platform analytical benchmarks using a protein standard sample, to allow users to gauge their proficiency.
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pasteur-02331018 , version 1 (24-10-2019)

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Daniel Donnelly, Catherine Rawlins, Caroline Dehart, Luca Fornelli, Luis Schachner, et al.. Best practices and benchmarks for intact protein analysis for top-down mass spectrometry. Nature Methods, 2019, 16 (7), pp.587-594. ⟨10.1038/s41592-019-0457-0⟩. ⟨pasteur-02331018⟩
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