Single-site phosphorylation within the His-tag sequence attached to a recombinant protein - Institut Pasteur Access content directly
Journal Articles Analytical Biochemistry Year : 2019

Single-site phosphorylation within the His-tag sequence attached to a recombinant protein

Abstract

We report the observation of single-site phosphorylation in a His-tag sequence N-terminally attached to a recombinant protein (UVI31+) in vitro. This modification was detected at position 23 at a serine residue of the His-tag sequence encoded by the vector pET28a. Furthermore, the phosphorylated tag sequence was found to be dephosphorylated by the action of alkaline phosphatases. The functional activity and dynamics of the protein carrying the His-tag sequence were unchanged after phosphorylation. The possibility of phosphorylation within the N-terminal His-tag demonstrates that care has to be taken upon analysis of post-translational modifications via mass spectrometry for recombinant protein expression strategies.
No file

Dates and versions

pasteur-02329972 , version 1 (23-10-2019)

Identifiers

Cite

Himanshu Singh, Deepshikha Verma, Benjamin Bardiaux. Single-site phosphorylation within the His-tag sequence attached to a recombinant protein. Analytical Biochemistry, 2019, 570, pp.62-64. ⟨10.1016/j.ab.2019.02.001⟩. ⟨pasteur-02329972⟩

Collections

PASTEUR CNRS
22 View
0 Download

Altmetric

Share

Gmail Facebook X LinkedIn More