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Journal Articles Journal of Cellular Biochemistry Year : 2019

Engagement of vimentin intermediate filaments in hypotonic stress

Abstract

Intermediate filaments (IFs) play a key role in the control of cell structure and morphology, cell mechano-responses, migration, proliferation, and apoptosis. However, the mechanisms regulating IFs organization in motile adhesive cells under certain physical/pathological conditions remain to be fully understood. In this study, we found hypo-osmotic-induced stress results in a dramatic but reversible rearrangement of the IF network. Vimentin and nestin IFs are partially depolymerized as they are redistributed throughout the cell cytoplasm after hypo-osmotic shock. This spreading of the IFs requires an intact microtubule network and the motor protein associated transportation. Both nocodazole treatment and depletion of kinesin-1 (KIF5B) block the hypo-osmotic shock-induced rearrangement of IFs showing that the dynamic behavior of IFs largely depends on microtubules and kinesin-dependent transport. Moreover, we show that cell survival rates are dramatically decreased in response to hypo-osmotic shock, which was more severe by vimentin IFs depletion, indicating its contribution to osmotic endurance. Collectively, these results reveal a critical role of vimentin IFs under hypotonic stress and provide evidence that IFs are important for the defense mechanisms during the osmotic challenge. K E Y W O R D S cytoplasmic redistribution, hypo-osmosis, intermediate filaments, osmotic stress, vimentin
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Dates and versions

pasteur-02138769 , version 1 (24-05-2019)

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Jian Li, Wei Gao, Yue Zhang, Fang Cheng, John E Eriksson, et al.. Engagement of vimentin intermediate filaments in hypotonic stress. Journal of Cellular Biochemistry, 2019, ⟨10.1002/jcb.28591⟩. ⟨pasteur-02138769⟩
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