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Book Sections Year : 2011

Viral Small RNA Cloning and Sequencing

Valérie Gausson

Abstract

At the current rate of technological progress, high-throughput sequencing of nucleic acids has become a commodity. These techniques are perfectly suitable for viral small RNAs sequencing and contribute to the understanding of many aspects of virus biology in the context of host-pathogen interaction. However, the generation of high quality data is still an issue and the preparation of small RNAs libraries that accurately reflect the viral siRNAs in the sample remains a challenge. In this chapter we describe how to clone and sequence libraries of viral small RNAs from infected insect samples (mosquito, drosophilidae, insect-derived cell lines).
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pasteur-02089924 , version 1 (04-04-2019)

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Valérie Gausson, Maria-Carla P Saleh. Viral Small RNA Cloning and Sequencing. Antiviral RNAi: Concepts, Methods, and Applications, 721, Springer, pp.107-122, 2011, Methods in Molecular Biology, 978-1-61779-037-9. ⟨10.1007/978-1-61779-037-9_6⟩. ⟨pasteur-02089924⟩

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