Bactericidal activity of sera from adolescents vaccinated with bivalent rLP2086 against meningococcal serogroup B outbreak strains from France. - Institut Pasteur Access content directly
Journal Articles Vaccine Year : 2017

Bactericidal activity of sera from adolescents vaccinated with bivalent rLP2086 against meningococcal serogroup B outbreak strains from France.

Muhamed-Kheir Taha
Julio Cesar Hawkins
  • Function : Author
Li Liberator
  • Function : Author
Lubomira Andrew
  • Function : Author
Li Hao
  • Function : Author
Thomas R. Jones
  • Function : Author
Lisa K. Mcneil
  • Function : Author
Robert E. O'Neill
  • Function : Author
John Perez
  • Function : Author
Kathrin U. Jansen
  • Function : Author
Annaliesa S. Anderson
  • Function : Author
  • PersonId : 1040436

Abstract

OBJECTIVES: Bivalent rLP2086 (Trumenba®; MenB-FHbp), composed of two factor H binding proteins (FHbps), is a vaccine approved in the United States for prevention of Neisseria meningitidis serogroup B (MnB) invasive meningococcal disease (IMD). Bactericidal activity of sera from subjects vaccinated with bivalent rLP2086 was assessed against MnB isolates from recent disease outbreaks in France. METHODS: MnB isolates from IMD cases were characterized by whole genome sequencing and FHbp expression was assessed using a flow cytometry-based assay. Sera from subjects (11-<19years old) vaccinated with bivalent rLP2086 at 0, 2, and 6months were evaluated. Bactericidal activity was measured in serum bactericidal assays using human complement (hSBAs). The response rate (RR) represents the percentage of subjects with an hSBA titer ⩾1:4. RESULTS: The six MnB outbreak isolates expressed diverse FHbp variants: A22, B03, B24 (two isolates), B44, and B228. FHbp expression levels ranged from 1309 to 8305 (mean fluorescence intensity units). The RR of preimmune sera from subjects was 7% to 27%. RRs increased for all isolates after each vaccine dose. After two doses, RRs ranged from 40% to 93%. After dose 3, RRs were ⩾73% for all isolates (range, 73%-100%). CONCLUSIONS: Each of the representative French outbreak isolates was killed by sera from subjects vaccinated with bivalent rLP2086. Vaccination elicited an immune response with bactericidal activity against these diverse isolates in a large proportion of subjects at risk. These results provide additional support for the licensure strategy of testing MnB strains expressing vaccine-heterologous FHbp variants in hSBAs and further illustrate the breadth of efficacy of this protein-based MnB vaccine.
No file

Dates and versions

pasteur-02013261 , version 1 (10-02-2019)

Identifiers

Cite

Muhamed-Kheir Taha, Julio Cesar Hawkins, Li Liberator, Ala-Eddine Deghmane, Lubomira Andrew, et al.. Bactericidal activity of sera from adolescents vaccinated with bivalent rLP2086 against meningococcal serogroup B outbreak strains from France.. Vaccine, 2017, 35 (11), pp.1530-1537. ⟨10.1016/j.vaccine.2017.01.066⟩. ⟨pasteur-02013261⟩

Collections

PASTEUR
38 View
0 Download

Altmetric

Share

Gmail Facebook X LinkedIn More