Diverged alleles of the Anopheles gambiae leucine-rich repeat gene APL1A display distinct protective profiles against Plasmodium falciparum. - Institut Pasteur Access content directly
Journal Articles PLoS ONE Year : 2012

Diverged alleles of the Anopheles gambiae leucine-rich repeat gene APL1A display distinct protective profiles against Plasmodium falciparum.

Inge Holm
  • Function : Author
  • PersonId : 746751
  • IdHAL : inge-holm
Michelle M. Riehle
  • Function : Author

Abstract

Functional studies have demonstrated a role for the Anopheles gambiae APL1A gene in resistance against the human malaria parasite, Plasmodium falciparum. Here, we exhaustively characterize the structure of the APL1 locus and show that three structurally different APL1A alleles segregate in the Ngousso colony. Genetic association combined with RNAi-mediated gene silencing revealed that APL1A alleles display distinct protective profiles against P. falciparum. One APL1A allele is sufficient to explain the protective phenotype of APL1A observed in silencing experiments. Epitope-tagged APL1A isoforms expressed in an in vitro hemocyte-like cell system showed that under assay conditions, the most protective APL1A isoform (APL1A(2)) localizes within large cytoplasmic vesicles, is not constitutively secreted, and forms only one protein complex, while a less protective isoform (APL1A(1)) is constitutively secreted in at least two protein complexes. The tested alleles are identical to natural variants in the wild A. gambiae population, suggesting that APL1A genetic variation could be a factor underlying natural heterogeneity of vector susceptibility to P. falciparum.
Fichier principal
Vignette du fichier
journal.pone.0052684.PDF (479.95 Ko) Télécharger le fichier
Loading...

Dates and versions

pasteur-02008328 , version 1 (05-02-2019)

Licence

Attribution

Identifiers

Cite

Inge Holm, Catherine Lavazec, Thierry Garnier, Christian Mitri, Michelle M. Riehle, et al.. Diverged alleles of the Anopheles gambiae leucine-rich repeat gene APL1A display distinct protective profiles against Plasmodium falciparum.. PLoS ONE, 2012, 7 (12), pp.e52684. ⟨10.1371/journal.pone.0052684⟩. ⟨pasteur-02008328⟩
28 View
83 Download

Altmetric

Share

Gmail Facebook X LinkedIn More