Differential entry of Botulinum Neurotoxin A into neuronal and intestinal cells: an ultrastructural approach - Institut Pasteur Access content directly
Journal Articles The Botulinum Journal Year : 2010

Differential entry of Botulinum Neurotoxin A into neuronal and intestinal cells: an ultrastructural approach

Abstract

Recombinant C-terminus (Hc) of botulinum neurotoxin A (BoNT/A) was used to monitor toxin entry into NG108-15 neuronal cells and m-ICcl2 intestinal cells by both fluorescence and electronic microscopy. BoNT/A Hc entered neuronal cells via a clathrin pathway and intestinal cells mainly via non-clathrin vesicles. Upon incubation at 4°C, BoNT/A Hc gold particles were mainly distributed to neuronal cell periphery and to a lower extent to m-ICcl2 cells. After incubation at 37°C, BoNT/A Hc mostly transited in coated vesicles in NG108-15 cells and in uncoated vesicles of m-ICcl2 cells, which support the transcytotic passage of the toxin.
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pasteur-01758348 , version 1 (04-04-2018)

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Aurélie Couesnon, Cesare Colasante, Jordi Molgó, Michel Popoff. Differential entry of Botulinum Neurotoxin A into neuronal and intestinal cells: an ultrastructural approach. The Botulinum Journal, 2010, 1 (4), ⟨10.1504/TBJ.2010.039113⟩. ⟨pasteur-01758348⟩

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