Role of Hydrophobins in Aspergillus fumigatus - Institut Pasteur Access content directly
Journal Articles Journal of Fungi Year : 2017

Role of Hydrophobins in Aspergillus fumigatus


Resistance of Aspergillus fumigatus conidia to desiccation and their capacity to reach the alveoli are partly due to the presence of a hydrophobic layer composed of a protein from the hydrophobin family, called RodA, which covers the conidial surface. In A. fumigatus there are seven hydrophobins (RodA–RodG) belonging to class I and III. Most of them have never been studied. We constructed single and multiple hydrophobin-deletion mutants until the generation of a hydrophobin-free mutant. The phenotype, immunogenicity, and virulence of the mutants were studied. RODA is the most expressed hydrophobin in sporulating cultures, whereas RODB is upregulated in biofilm conditions and in vivo. Only RodA, however, is responsible for rodlet formation, sporulation, conidial hydrophobicity, resistance to physical insult or anionic dyes, and immunological inertia of the conidia. None of the hydrophobin plays a role in biofilm formation or its hydrophobicity. RodA is the only needed hydrophobin in A. fumigatus, conditioning the structure, permeability, hydrophobicity, and immune-inertia of the cell wall surface in conidia. Moreover, the defect of rodlets on the conidial cell wall surface impacts on the drug sensitivity of the fungus.


Fichier principal
Vignette du fichier
jof-252503.pdf (4.5 Mo) Télécharger le fichier
jof-252503-supplementary.pdf (1.47 Mo) Télécharger le fichier
Origin Publication funded by an institution

Dates and versions

pasteur-01673574 , version 1 (30-12-2017)




Isabel Valsecchi, Vincent Dupres, Emmanuel Stephen-Victor, J. Iñaki Guijarro, John Gibbons, et al.. Role of Hydrophobins in Aspergillus fumigatus. Journal of Fungi, 2017, 4 (1), pp.2. ⟨10.3390/jof4010002⟩. ⟨pasteur-01673574⟩
285 View
174 Download



Gmail Mastodon Facebook X LinkedIn More