Purification of G1 daughter cells from different Saccharomycetes species through an optimized centrifugal elutriation procedure. - Institut Pasteur Access content directly
Journal Articles Yeast Year : 2014

Purification of G1 daughter cells from different Saccharomycetes species through an optimized centrifugal elutriation procedure.

Abstract

Centrifugal elutriation discriminates cells according to their sedimentation coefficients, generating homogeneous samples well suited for genomic comparative approaches. It can, for instance, isolate G1 daughter cells from a Saccharomyces cerevisiae unsynchronized population, alleviating ageing and cell-cycle biases when conducting genome-wide/single-cell studies. The present report describes a straightforward and robust procedure to determine whether a cell population of virtually any yeast species can be efficiently elutriated, while offering solutions to optimize success. This approach was used to characterize elutriation parameters and S-phase progression of four yeast species (S. cerevisiae, Candida glabrata, Lachancea kluyveri and Pichia sorbitophila) and could theoretically be applied to any culture of single, individual cells.

Domains

Genetics
Fichier principal
Vignette du fichier
Marbouty et al Yeast 2012.pdf (1.4 Mo) Télécharger le fichier
Origin : Files produced by the author(s)
Loading...

Dates and versions

pasteur-01420008 , version 1 (05-05-2017)

Licence

Attribution

Identifiers

Cite

Martial Marbouty, Caroline Ermont, Bernard Dujon, Guy-Franck Richard, Romain Koszul. Purification of G1 daughter cells from different Saccharomycetes species through an optimized centrifugal elutriation procedure.. Yeast, 2014, 31 (5), pp.159-166. ⟨10.1002/yea.3005⟩. ⟨pasteur-01420008⟩
155 View
529 Download

Altmetric

Share

Gmail Facebook X LinkedIn More