Variation in copy number of the 28S rDNA of Aspergillus fumigatus measured by droplet digital PCR and analog quantitative real-time PCR - Institut Pasteur Access content directly
Journal Articles Journal of Microbiological Methods Year : 2016

Variation in copy number of the 28S rDNA of Aspergillus fumigatus measured by droplet digital PCR and analog quantitative real-time PCR

Abstract

Droplet digital PCR (ddPCR) after DNA digestion yielded a 28S rDNA copy number of 61 to 86 copies/genome when testing 10 unrelated Aspergillus fumigatus isolates, higher than with quantitative PCR. Unfortunately, ddPCR after DNA digestion did not improve the sensitivity of our PCR assay when testing serum patients with invasive aspergillosis.
No file

Dates and versions

pasteur-01405204 , version 1 (29-11-2016)

Identifiers

Cite

Alexandre Alanio, Aude Sturny-Leclère, Marion Benabou, Nicolas Guigue, Stéphane Bretagne. Variation in copy number of the 28S rDNA of Aspergillus fumigatus measured by droplet digital PCR and analog quantitative real-time PCR. Journal of Microbiological Methods, 2016, 127, pp.160--163. ⟨10.1016/j.mimet.2016.06.015⟩. ⟨pasteur-01405204⟩
67 View
0 Download

Altmetric

Share

Gmail Facebook X LinkedIn More