Simultaneous assessment of autophagy and apoptosis using multispectral imaging cytometry. - Institut Pasteur Access content directly
Journal Articles Autophagy Year : 2011

Simultaneous assessment of autophagy and apoptosis using multispectral imaging cytometry.

Abstract

Multiple stress pathways result in the induction of autophagy and apoptosis. Current methods (e.g., protein gel blot, microscopy) do not offer quantitative single-cell resolution, thus making it difficult to discern if these pathways are mutually exclusive or, in some situations, cooperative in executing cell death. We report a novel method that enables high-throughput, high-content assessment of LC3 puncta and caspase-3 cleavage at the single cell level.

Dates and versions

pasteur-01402064 , version 1 (24-11-2016)

Identifiers

Cite

Claire de La Calle, Pierre-Emmanuel Joubert, Helen K W Law, Milena Hasan, Matthew L Albert. Simultaneous assessment of autophagy and apoptosis using multispectral imaging cytometry.. Autophagy, 2011, 7 (9), pp.1045-51. ⟨10.4161/auto.7.9.16252⟩. ⟨pasteur-01402064⟩

Collections

PASTEUR ATP-IMMUN
26 View
0 Download

Altmetric

Share

Gmail Facebook X LinkedIn More